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All Journal Bioscientist : Jurnal Ilmiah Biologi
Caterina Hidayati
State University of Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Phenotypic and Genomic Detection of Salmonella spp. in Broiler Chicken Meat Across Distribution Chains in Surabaya, Indonesia Caterina Hidayati; Isnawati; Wahyu Setyarini; Radita Yuniar Arizandy
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20420

Abstract

This study aimed to investigate Salmonella spp. contamination in broiler chicken meat obtained from traditional markets, modern markets, and poultry slaughterhouses in Surabaya, East Java, using phenotypic and genomic approaches. A total of 15 broiler chicken meat samples were analyzed. Phenotypic identification was performed through colony morphology observation, Gram staining, and biochemical testing, while genomic confirmation was conducted using Polymerase Chain Reaction (PCR) targeting the invA gene. The results showed that two samples (13.33%) obtained from traditional markets were positive for Salmonella spp., whereas all samples collected from modern markets and poultry slaughterhouses tested negative. These findings indicate variation in contamination levels among different distribution sources, with a higher risk of Salmonella spp. contamination in broiler chicken meat sold in traditional markets. Therefore, strengthened surveillance and improved hygiene and sanitation practices are essential to ensure food safety and protect public health. This study provides comparative evidence of Salmonella spp. contamination across different broiler chicken distribution channels through the integration of phenotypic identification and molecular confirmation.
Co-Authors Isnawati Radita Yuniar Arizandy Wahyu Setyarini