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All Journal INDOGENIUS Cakrawala: Jurnal Pengabdian Masyarakat Global
Rizki Annisa Ramadhani
Universitas Negeri Semarang
Agriculture, Biological Sciences & Forestry Humanities Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Nursing Social Sciences

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Article Review: Identification of Secondary Metabolites Using the TLC-Densitometry Method Rizki Annisa Ramadhani; Hilmia Mufida; Yola Asri Paramitha; Muhammad Zaki Hamzah; Neza Ardelia Putri; Neysa Husna Luthfiyah; Zahra Putri Ramadhani; Dini Indriyanti
INDOGENIUS Vol 5 No 2 (2026): INDOGENIUS
Publisher : Department of Publication of Inspirasi Elburhani Foundation Desa. Pamokolan, Kecamatan Cihaurbeuti, Kabupaten Ciamis, Provinsi Jawa Barat, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/igj.v5i2.968

Abstract

Background & Objective: This review article discusses the identification and quantification of secondary metabolites using densitometric thin-layer chromatography (TLC) based on 24 journal articles published over the past ten years. The compounds analyzed include quercetin, catechins and their derivatives (EGCG), phenolic compounds, flavonoids, and various other secondary metabolites. Method: Based on the overall data, densitometric TLC is an effective method for the identification and quantification of secondary metabolites because it provides good separation, clear Rf values, and stable quantification results across various natural material matrices. Result: The results of the study indicate that silica gel 60 F254 and GF254 are the most commonly used stationary phases, while variations in the mobile phase are the primary factors influencing Rf values and separation resolution. In the quercetin group, Rf values ranged from 0.26 to 0.89, with the highest concentration in meniran at 3.5% and the lowest in cocoa beans at 0.115%. Conclusion: Analysis of catechins showed Rf values ranging from 0.22 to 0.60, with the highest content in avocado seeds at 25.55% and gambir at 25.50%, while EGCG in green tea had an Rf value of 0.21 and a content of 3.33%. Phenolic and flavonoid compounds exhibited Rf values ranging from 0.24 to 0.94, influenced by differences in polarity and solvent composition. Other secondary metabolites such as syringic acid, kaempferol, chlorogenic acid, eugenol, beta-sitosterol, oleanolic acid, ursolic acid, myricetin, and rutin exhibited migration patterns consistent with their chemical properties.
Peningkatan Pemahaman Santri dan Pengelola Pesantren dalam Mengolah dan Mengonsumsi Produk Serbuk Kelor-Jahe di Pondok Pesantren As-Salamah Betahwalang Kabupaten Demak Anggita Dipika Wulandari; Rifda Naufa Lina; Matheus Prayoga Claus; Rahmawati salsa dinurrosifa; Laelatul Husniyah; Rizki Annisa Ramadhani; Setya Wahono; Aldo Octavian Ramadhan Saputra
Cakrawala: Jurnal Pengabdian Masyarakat Global Vol. 5 No. 3 (2026): Agustus: Cakrawala: Jurnal Pengabdian Masyarakat Global
Publisher : Universitas 45 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30640/cakrawala.v5i3.7865

Abstract

Islamic boarding schools are educational environments characterized by communal living, presenting unique challenges for health maintenance. Factors such as high-density living, shared habits, and limited understanding of nutrition and the use of herbal ingredients can impact efforts to maintain the health of the students. Moringa oleifera and Zingiber officinale are local ingredients with potential for use in food diversification and herbal beverages. This community service activity aimed to enhance the understanding and skills of the students and administrators at the As-Salamah Betahwalang Islamic Boarding School regarding the utilization of Moringa and ginger by processing them into instant powder products. The activity employed an educational and participatory approach, encompassing education on balanced nutrition and immunity, an introduction to the benefits of Moringa and ginger, plant cultivation practices, demonstrations and training on instant powder production and packaging, and evaluation via pre- and post-tests. Participants actively engaged in discussions, demonstrations, processing practices, and cultivation activities. A Moringa-ginger instant powder product was successfully produced through a process involving ingredient preparation, boiling Moringa, processing ginger, filtering, heating, adding sugar, stirring until powder formed, and packaging. The activity also established Moringa and ginger cultivation practices as a strategy to ensure a sustainable supply of raw materials. This program provided the partners with a foundation of knowledge and skills for utilizing local herbal ingredients to create practical products with the potential for independent and sustainable development.
Co-Authors Aldo Octavian Ramadhan Saputra Anggita Dipika Wulandari Dini Indriyanti Hilmia Mufida Laelatul Husniyah Matheus Prayoga Claus Muhammad Zaki Hamzah Neysa Husna Luthfiyah Neza Ardelia Putri Rahmawati Salsa Dinurrosifa Rifda Naufa Lina Setya Wahono Yola Asri Paramitha Zahra Putri Ramadhani