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All Journal TENTIRO: Jurnal Pendidikan dan Kesehatan Jurnal Analis Kesehatan Kendari
Anak Agung Ayu Eka Cahyani
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PERBEDAAN HASIL PEMERIKSAAN LEUKOSIT URINE MENGGUNAKAN URINE ANALYZER DENGAN SEDIMEN URINE Lydia Erdi Putri; Anak Agung Ayu Eka Cahyani; Putu Ayu Parwati
TENTIRO: Jurnal Pendidikan dan Kesehatan Vol. 3 No. 1 (2026): Mei || TENTIRO: Jurnal Pendidikan dan Kesehatan
Publisher : PT. GIAT KONSELING NUSANTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70420/

Abstract

Urinalysis is a routine urine examination in the laboratory that includes macroscopic, chemical, and microscopic (urine sediment) analysis. One of the most commonly found elements in urine sediment is leukocytes. Until now, the gold standard for urine sediment examination has been manual microscopy. However, with technological advancements, many laboratory personnel now use automated methods such as the Cybow Reader, which allows for quicker analysis.The aim of this study is to determine the difference in urine leukocyte results between the Cybow Reader and the manual microscopic (sediment) method. This research is experimental in nature. The population of the study consisted of all patients undergoing urinalysis at Seger Waras Hospital in May 2025. Using accidental sampling, a total of 20 samples were collected. The urinary leukocyte count obtained using the urine analyzer method showed results of 25/ul in 17 samples (85%), 100/ul in 1 sample (5%), and 500/ul in 2 sampels (10%). Meanwhile, the urinary leukocyte count using the urine sediment method showed results of +1 in 17 samples (85%),+2 in 1 sample (5%) and +3 in 2 samples (10%).The data were analyzed using the Shapiro-Wilk normality test, followed by the Kruskal-Wallis test. The Kruskal-Wallis test yielded a p-value of 0.00, indicating that there is a significant difference in urine leukocyte results between the urine analyzer and the sediment method.
HUBUNGAN HASIL PEMERIKSAAN SGOT DENGAN SGPT PADA PASIEN DEMAM BERDARAH DI LABORATORIUM PATOLOGI KLINIK RSUD WANGAYA KOTA DENPASAR I Made Sumertayasa; Anak Agung Ayu Eka Cahyani; Putu Ayu Parwati
TENTIRO: Jurnal Pendidikan dan Kesehatan Vol. 3 No. 1 (2026): Mei || TENTIRO: Jurnal Pendidikan dan Kesehatan
Publisher : PT. GIAT KONSELING NUSANTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70420/

Abstract

Dengue Fever is an acute febrile illness caused by the dengue virus and transmitted by the Aedes aegypti mosquito. The target organ of the dengue virus is the liver, and mild transaminitis is common in patients with dengue virus infection. Serum Glutamic Oxaloacetic Transaminase (SGOT) Serum glutamic pyruvate transaminase (SGPT) levels usually return to normal levels within 1-2 weeks. The pathogenesis of acute liver failure in patients with dengue is thought to be caused by the direct effect of the dengue virus on liver cells or due to an abnormal immune response to the dengue virus. This study was conducted with secondary data collected from January to June 2025. This type of study is a correlational study with a sample size of 54 people. The results of the SGOT examination in dengue fever patients with a minimum value of 37 U/L, a maximum of 96 U/L, a mean of 71.24 U/L and an SD of 13.67 U/L. The results of the SGPT examination of dengue fever patients obtained a minimum value of 35 U/L, a maximum of 69 U/L, a mean of 53.54 U/L and SD of 7.14 U/L. The results of the Pearson Product Moment correlation test obtained a p-value of 0.000 (p-value <0.05). This indicates a relationship between the results of the SGOT examination and SGPT in DHF patients. The increase in SGOT and SGPT in dengue patients is a result of liver cell injury considering that the dengue virus attacks the reticuloendothelial system of the host. This liver injury is due to the direct effect of the virus, but can also be caused by the host's immune response. One of these is the involvement of CD4+ cytotoxic T cells. This interaction causes the destruction of cells that present viral antigens and cells that do not present antigens in surrounding cells that also express Fas.
ANALISIS PERBEDAAN KADAR ALBUMIN SEBELUM DAN SESUDAH HEMODIALISA PADA PASIEN GAGAL GINJAL KRONIK DI LABORATORIUM DI RUMAH SAKIT SANTA ELISABETH Fitri Agnes Simatupang; Anak Agung Ayu Eka Cahyani; Moh Fairuz Abadi
TENTIRO: Jurnal Pendidikan dan Kesehatan Vol. 3 No. 1 (2026): Mei || TENTIRO: Jurnal Pendidikan dan Kesehatan
Publisher : PT. GIAT KONSELING NUSANTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70420/

Abstract

Chronic kidney failure (CKF) is a progressive decline in kidney function so that it connot maintain hemostasis in the body, in order to maintain hemostasis in the body, hemodialysis is performed on patients with kidney failure, to replce kidney function. Albumin examination is serum or plasma taken from venous blood. The purpose of this study was to analyze albumin levels in patients with chronic kidney failure before and after hemodialysis at Santa Elisabe Hospital Medan. This research method uses non-probability sampling quota. The population in this study was 176 patients with a sample size of 64 respondents. The results of the study showed that albumin levels before hemodialysis in patients with chronic kidney failure had an average of 2.8484 g/dL. Albumin levels after hemodialysis had an average of 3.1719 g/dL, the results of the statistical analysis obtained a p-value of 0.000. this shows that the p-value<0.005, which means that H0 is rejected and H1 is accepted, so it can be concluded that there is a difference in albumin levels before and after hemodialysis in patients with kidney failure.
Pengaruh Lama Fiksasi Neutral Buffered Formalin 10% Terhadap Kualitas Hasil Pewarnaan Hematoksilin Eosin Jaringan Mioma Uteri di RSUD Wangaya Ketut Devita; Anak Agung Ayu Eka Cahyani; Putu Gede Subhaktiyasa
Jurnal Analis Kesehatan Kendari Vol. 8 No. 2 (2026): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 8 (2) June 2026)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v8i2.427

Abstract

Uterine fibroids are benign tumors originating from the smooth muscle of the uterus, and their diagnosis can be established through histopathological examination using Hematoxylin and Eosin (H&E) staining. One of the important stages in tissue preparation is the fixation process using 10% Neutral Buffered Formalin (NBF), as the duration of fixation may affect the quality of tissue staining. This study aimed to determine the effect of 10% NBF fixation duration on the quality of Hematoxylin and Eosin (H&E) staining in uterine fibroid tissue with fixation times of 12, 24, and 48 hours.This study employed an analytical experimental design and was conducted at Wangaya Regional General Hospital from January to February. Data were analyzed using the Shapiro–Wilk normality test, which showed that the data were not normally distributed; therefore, the Kruskal–Wallis test was subsequently performed. The results showed a p-value of 0.006, indicating a significant difference among the groups. Post hoc analysis using the Mann–Whitney test demonstrated significant differences between the 12-hour and 24-hour fixation groups (p = 0.047) and between the 12-hour and 48-hour fixation groups (p = 0.007), whereas no significant difference was found between the 24-hour and 48-hour fixation groups. The results indicate that the duration of 10% NBF fixation affects the quality of Hematoxylin and Eosin (H&E) staining in uterine fibroid tissue, in which fixation for 24–48 hours provides better staining quality than fixation for 12 hours. This study is expected to serve as a reference for determining tissue fixation duration in routine histopathological examinations and for further research regarding tissue fixation processes and the quality of Hematoxylin and Eosin (H&E) staining. Keywords: Fixation Time, 10% Neutral Buffered Formalin, H&E Staining.
Co-Authors Fitri Agnes Simatupang I Made Sumertayasa Ketut Devita Lydia Erdi Putri Moh Fairuz Abadi Putu Ayu Parwati Putu Gede Subhaktiyasa