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Akhmad
Universitas Negeri Makassar
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KARAKTERISASI ISOLAT BAKTERI RIZOSFER JAGUNG HIBRIDA (Zea mays L.) PADA TANAH MASAM Akhmad
Jurnal Biogenerasi Vol. 10 No. 4 (2025): Volume 10 nomor 4 tahun 2025 Terbit Oktober-Desember 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/cq4z0s16

Abstract

Acidic soil is one of the major constraints in maize cultivation because low soil pH can increase the solubility of Al and Fe, inhibit root growth, and reduce nutrient availability, particularly phosphorus. Rhizosphere bacteria have the potential to support plant growth through various mechanisms; therefore, the exploration of local bacterial isolates from acidic soil environments is important. This study aimed to isolate and characterize rhizosphere bacteria associated with hybrid maize grown in acidic soil in Moncongloe Village, Maros Regency. Bacterial isolation was carried out using serial dilution and cultivation on Nutrient Agar (NA) medium, followed by colony purification and characterization based on colony morphology, Gram reaction using 3% KOH, and catalase activity using 3% H₂O₂. The results showed that 14 bacterial isolates were obtained from maize rhizosphere samples for further characterization. All isolates showed a Gram-positive reaction, while the catalase test revealed that 11 isolates (78.57%) were catalase-positive and 3 isolates (21.43%) were catalase-negative. Differences in morphological and basic biochemical characteristics indicate the diversity of rhizosphere bacterial isolates capable of growing under acidic soil conditions. These isolates have the potential to serve as local microbial candidates for biofertilizer development; however, further studies are required to evaluate their PGPR functional traits, biosafety, and molecular identification.
Keanekaragaman Isolat Bakteri Rizosfer Tanaman Jagung (Zea mays L.) pada Tanah Masam di Kabupaten Maros Akhmad
Jurnal Biogenerasi Vol. 10 No. 4 (2025): Volume 10 nomor 4 tahun 2025 Terbit Oktober-Desember 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/w0nnv838

Abstract

This study aimed to the diversity of rhizosphere bacterial isolates associated with maize (Zea mays L.) cultivated in acidic soils of Maros Regency, South Sulawesi Province. Acidic soils characterized by low pH and limited nutrients, but the ability of microorganisms can adapt according to their habitat. However, microorganisms, particularly rhizosphere bacteria, exhibit remarkable adaptability to such conditions. The research employed serial dilution and culture on Nutrient Agar (NA) media to isolate bacteria from rhizosphere soil samples. A total of 14 bacterial isolates were toward will different morphological characteristics. All bacterial isolates found are Gram-positive bacteria. The findings reveal a high diversity of rhizosphere bacterial isolates, suggesting their potential role in enhancing plant growth as biofertilizers. Further research is necessary to evaluate the physiological and molecular potential of these bacterial isolates in nitrogen fixation, phosphate solubilization, and the production of plant growth hormones. Additionally, exploring the potential application of these bacteria as biocontrol agents or biofertilizers could contribute to improving maize productivity in acidic soils
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