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All Journal Jurnal Ilmu Kedokteran dan Kesehatan Journal of Health and Nutrition Research
Stephani Linggawan
Faculty of Medicine, Universitas Wijaya Kusuma Surabaya, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Correlation Between Platelet-to-Lymphocyte Ratio and Low-Density Lipoprotein Cholesterol Levels in Patients With Coronary Heart Disease Budiono Raharjo; Halimah Siahaan; Muhammad Sungging Pradana; Halik Wijaya; Avena Athalia Alim; Yohanes Timothy Raharjo; Stephani Linggawan; Anton Sumarpo
Journal of Health and Nutrition Research Vol. 5 No. 1 (2026)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jhnresearch.v5i1.951

Abstract

Coronary heart disease (CHD) remains a leading cause of mortality worldwide and is primarily driven by atherosclerosis, a pathological process in which dyslipidemia and chronic inflammation play central roles. Elevated low-density lipoprotein (LDL) cholesterol is a well-established risk factor for atherogenesis, contributing to lipid accumulation within the arterial wall and subsequent plaque formation. In parallel, inflammatory mechanisms are critically involved in plaque progression and instability, underscoring the importance of inflammatory biomarkers in cardiovascular research. The platelet-to-lymphocyte ratio (PLR) has emerged as a simple and cost-effective hematological marker that reflects systemic inflammation and prothrombotic activity. Several studies have reported an association between elevated PLR and adverse cardiovascular outcomes. However, the relationship between PLR and lipid parameters, particularly LDL cholesterol, remains unclear. Previous findings have been inconsistent, and limited evidence is available regarding this association in patients with stable and medically treated CHD. Given these uncertainties, further investigation is warranted to clarify the relationship between inflammatory markers and lipid profiles in stable CHD populations. Therefore, this study aimed to evaluate the correlation between the platelet-to-lymphocyte ratio and LDL cholesterol levels in patients with stable coronary heart disease.
Pengaruh Ekstrak Etanol Gelidium Latifolium Terhadap Ekspresi Protein EGFR Pada Kanker Serviks: Studi In Vitro Pada Sel HeLa Salwa Afifah Maharani; Feliks Richard Zen Chandra; Athallah Jemila Maharani Balqis; Waode Nurul Iman Albaiti; Budiono Raharjo; Deddy Hartanto; Farida Anggraini Soetedjo; Stephani Linggawan; Yohanes Timothy Raharjo; Anton Sumarpo
Jurnal Ilmu Kedokteran dan Kesehatan Vol 13, No 8 (2026): Volume 13 Nomor 8
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jikk.v13i8.25641

Abstract

Kanker serviks tetap menjadi masalah kesehatan global dengan tingkat insidensi yang tinggi di Indonesia, sementara akses terhadap terapi konvensional masih terbatas. Ekspresi berlebih (overexpression) dari epidermal growth factor receptor (EGFR) berperan penting dalam patogenesis kanker serviks yang diinduksi oleh infeksi human papillomavirus (HPV). Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol Gelidium latifolium terhadap ekspresi protein EGFR pada galur sel HeLa secara in vitro. Ekstraksi dilakukan melalui maserasi bertingkat menggunakan etanol 96%. Sel HeLa dikultur dalam medium DMEM dan diberi perlakuan ekstrak dengan konsentrasi 0 (kontrol), 200, 300, dan 400 µg/mL selama 24 dan 48 jam. Viabilitas sel dinilai menggunakan uji CCK-8, sedangkan ekspresi protein EGFR dianalisis melalui teknik imunofluoresensi menggunakan antibodi anti-EGFR terkonjugasi FITC dan pewarnaan inti DAPI. Hasil penelitian menunjukkan bahwa ekstrak tersebut menekan viabilitas sel secara dose-dependent (bergantung pada dosis), namun tidak bergantung waktu dengan nilai R²=0,9967 per 24 jam dan R²=0,9986 per 24 jam (p=0,1991), dengan nilai IC50 sebesar 276,8 µg/mL (24 jam) dan 246,6 µg/mL (48 jam). Selanjutnya, ekspresi protein EGFR menurun secara signifikan seiring dengan peningkatan konsentrasi ekstrak, menunjukkan tingkat ekspresi sebesar 72,4% (200 µg/mL, p0,05), 48,7% (300 µg/mL, p0,01), dan 25,3% (400 µg/mL, p0,001) dibandingkan dengan kontrol. Sebagai kesimpulan, ekstrak etanol Gelidium latifolium secara efektif menurunkan ekspresi protein EGFR pada sel HeLa secara in vitro, menyoroti potensinya untuk dikembangkan sebagai terapi adjuvan alami yang berbiaya rendah dan berkelanjutan untuk kanker serviks.
Co-Authors Anton Sumarpo Anton Sumarpo Athallah Jemila Maharani Balqis Avena Athalia Alim Budiono Raharjo Deddy Hartanto Farida Anggraini Soetedjo Feliks Richard Zen Chandra Halik Wijaya Halimah Siahaan Muhammad Sungging Pradana Salwa Afifah Maharani Waode Nurul Iman Albaiti Yohanes Timothy Raharjo Yohanes Timothy Raharjo