Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal VALENSI
Niken Istikhari Muslihah
Department of Chemistry, Faculty of Mathematics and Natural Science, Jenderal Soedirman University
Chemistry

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Kinetic Behavior and Stability of Alginate-Immobilized Urease from Green Bean Seeds (Phaseolus vulgaris) Zusfahair Zusfahair; Sifa Nurfadillah Setiawan; Dian Riana Ningsih; Bilalodin Bilalodin; Amin Fatoni; Niken Istikhari Muslihah
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 12, No. 1, May 2026
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v12i1.49961

Abstract

The industrial application of urease is limited due to its low reusability, which can be overcome by immobilization. Furthermore, the utilization of urease from plants such as mung bean seeds, which is affordable and readily available, offers a sustainable alternative to commercial enzymes. This study aimed to immobilize urease extracted from green bean seeds using an alginate matrix and to evaluate its kinetic and stability characteristics. Urease activity was measured at 500 nm using the Nessler method. Kinetic analysis showed that immobilization decreased  Vmax from 0.674 to 0.223 M min-1 and increased KM from 0.005169 to 0.006755 M. After incubation at 35 oC for 180 min, immobilized urease showed higher relative activity (52.26%) than free enzyme (42.82%). Stored at 4 oC for 9 days, the enzyme preserved 56.68% of its relative activity. The immobilized urease displayed the ability to be reused up to five cycles. SEM analysis showed morphological changes in the beads after enzyme incorporation, while EDX confirmed an increase in the elemental composition of the immobilized beads. This study shows that urease from green bean seeds can be effectively immobilized in an alginate matrix to produce a stable, reusable, low-cost biocatalyst with potential for application in urea biosensors.
Co-Authors Amin Fatoni Bilalodin Bilalodin Dian Riana Ningsih Sifa Nurfadillah Setiawan Zusfahair Zusfahair