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All Journal JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION)
Fatwa Annisa Yustisia
Universitas Gadjah Mada, Indonesia
Agriculture, Biological Sciences & Forestry Environmental Science

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Molecular Characterization and Mechanical Transmission of a Papaya Ringspot Virus Isolate from Sleman Regency, Indonesia Meyrin Novia Vadilah; Fhilia Raga Cipta Wida; Rossa Wulandari; Alimah Nur Hasanah; Fatwa Annisa Yustisia; Carreista Ashila Shafa; Fadzilah Nur Hidayah; Hanum Nilamsari; Adyatma Irawan Santosa
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 10 No. 1 (2026): June 2026
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.10.1.42-55.2026

Abstract

Viral diseases severely affect papaya (Carica papaya Linnaeus) production in Indonesia, but information on papaya ringspot virus (PRSV) isolates from severe outbreaks in Sleman Regency remains limited. This study aimed to identify the causal virus and evaluate its mechanical transmissibility to selected cucurbit hosts. A composite leaf sample from five symptomatic papaya plants was tested for potyvirus and begomovirus using universal primers. The potyvirus amplicon was sequenced and analyzed using BLASTn, phylogenetic analysis, and pairwise nucleotide identity analysis based on a 462 bp trimmed partial coat protein region. Mechanical transmission was tested on cucumber (Cucumis sativus), melon (Cucumis melo), and bitter gourd (Momordica charantia), followed by symptom observation and RT-PCR confirmation. This study identified identified the virus as PRSV-P, registered as PRSV-SMN-TJ, accession number PX927038. Disease incidence after inoculation ranged from 50% to 100%. Phylogenetic and nucleotide identity analyses placed the PRSV-SMN-TJ isolate within the same cluster as Indonesian and Thailand PRSV isolates. This study was based on a single composite field sample and a limited host transmission assay; therefore, broader sampling, complete diagnostic controls, and quantitative pathogenicity assessment are needed. Nevertheless, these findings provide baseline information for PRSV surveillance and management planning in papaya-growing areas.
Co-Authors Adyatma Irawan Santosa Alimah Nur Hasanah Carreista Ashila Shafa Fadzilah Nur Hidayah Fhilia Raga Cipta Wida Hanum Nilamsari Meyrin Novia Vadilah Rossa Wulandari