The World Health Organization (WHO) notes that 40% of maternal deaths in developing countries are related to chronic energy deficiency. The prevalence of Chronic Energy Deficiency (CED) among pregnant women in Indonesia is 16.9%, a figure that has not yet reached the target of 10% set by the Ministry of Health of the Republic of Indonesia in the 2024 Ministry of Health Strategic Plan. One method of education to prevent CED is nutrition education which can be conducted face-to-face accompanied by the provision of booklets. This research analyzes the differences in knowledge and application of protein consumption among pregnant women before and after receiving nutrition education using booklets, carried out from March to April. This type of research uses a one group pretest-posttest approach before and after the intervention. The results of this study indicate that before being provided with nutrition education using a booklet, pregnant women had a lowest knowledge score of 47 and the highest score of 80, with an average score of 66.74, and the majority of mothers had an inappropriate protein consumption application. After being provided with nutrition education using a booklet, pregnant women had a lowest knowledge score of 60 and the highest score of 93, with an average score of 76.62, and the majority of mothers had an appropriate protein consumption application. The Wilcoxon test results showed a p-value of 0.000 < 0.05, indicating a difference in knowledge and application of protein consumption among pregnant women before and after nutrition education was provided using booklet media. The researcher hopes that this study can be beneficial for the development of knowledge, pregnant women, healthcare services, and the community.