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All Journal JURNAL FARMASIMED (JFM) Indonesia Natural Research Pharmaceutical Journal (INRPJ)
Theodorus Rexa Handoyo
Universitas 17 Agustus 1945 Jakarta
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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Development and Method Validation of HPLC Method as an Key Method in the Analysis of Olopatadine HCl Theodorus Rexa Handoyo; Violetta Djohansah; Windy Annisa Arbah
Jurnal FARMASIMED (JFM) Vol 8 No 1 (2025): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/68pp3f94

Abstract

Olopatadine hydrochloride (HCl) is a widely used second-generation antihistamine, and its accurate quantification is critical to ensure product quality and therapeutic efficacy. Reliable analytical methods are therefore essential for regulatory compliance and routine quality control. This study aimed to develop and validate a simple and robust high-performance liquid chromatography (HPLC) method for the determination of olopatadine HCl in pharmaceutical samples. The method was validated in accordance with USP <1225>, AOAC, and ICH Q2(R1) guidelines, with evaluation of key parameters including linearity, accuracy, precision, selectivity, sensitivity, and system suitability. The validation outcomes confirmed that the method provided strong linearity across the tested range, acceptable recovery values, excellent repeatability, and sufficient sensitivity for low-level detection. Overall, the developed HPLC method proved to be accurate, precise, selective, and reliable. Its simplicity and cost-effectiveness make it particularly suitable for routine quality control of olopatadine HCl in research and pharmaceutical industry laboratories.
In Silico Study of Kaempferol Targeting MMPs and COX-2 for Anti-Aging and Wound Healing Theodorus Rexa Handoyo
INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL Vol 10, No 2 (2025)
Publisher : Fakultas Farmasi Universitas 17 Agustus 1945 Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52447/inrpj.v10i2.9214

Abstract

Skin aging and impaired wound healing are complex biological processes involving chronic inflammation and excessive degradation of the extracellular matrix. Matrix metalloproteinases (MMP-1, MMP-3, and MMP-9) and cyclooxygenase-2 (COX-2) play crucial roles in these processes and are considered important molecular targets for anti-aging and wound healing therapies. Kaempferol is a natural flavonoid compound known for its antioxidant and anti-inflammatory activities; however, its molecular interaction mechanisms with these targets remain incompletely understood. This study aimed to evaluate the potential of kaempferol as an anti-aging and wound healing agent through a molecular docking approach targeting MMP-1, MMP-3, MMP-9, and COX-2. Molecular docking simulations were performed using Molegro Virtual Docker 6.0, while ligand-protein interactions were visualized and analyzed using BIOVIA Discovery Studio Visualizer v.21.1.0.20298. The docking results indicated that kaempferol exhibited favorable binding affinities toward all target proteins, with the strongest interactions observed for MMP-9 and COX-2. Kaempferol formed conventional hydrogen bonds as well as hydrophobic and aromatic interactions within the active sites of the proteins. In conclusion, kaempferol demonstrates potential as a multitarget natural compound for anti-aging and wound healing applications, although further in vitro and in vivo studies are required to validate its biological activity.
Co-Authors Violetta Djohansah Windy Annisa Arbah