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All Journal Journal of Marine and Coastal Science Jurnal Pengabdian kepada Masyarakat
Chandra Trisna Pangestu
Universitas Airlangga
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Social Sciences Other

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Molecular Identification of Elasmobranch Fin Products Traded Online Using DNA Barcoding Chandra Trisna Pangestu; Heru Pramono; Sapto Andriyono
Journal of Marine and Coastal Science Vol. 15 No. 2 (2026): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v15i2.89543

Abstract

The trade in dried shark and ray fins results in the loss of diagnostic morphological characteristics, making visual species identification difficult, particularly in online transactions. This study aims to identify elasmobranch species in dried fin products marketed online using a DNA barcoding approach based on the cytochrome c oxidase subunit I (COI) gene. The study was a descriptive-exploratory analysis of two dried fin samples obtained from Facebook listings, namely BPKUAFB1 and BPKUAFB2. DNA was extracted from fin tissue, the COI gene was amplified using universal LCO/HCO primers, and PCR products were verified via electrophoresis prior to Sanger sequencing. The sequencing results were analyzed using BLASTN against the GenBank/NCBI database and validated by phylogenetic analysis using the Neighbor-Joining method with the Kimura 2-parameter model in MEGA 12. Both samples showed 100% query coverage and 99.84% identity to the GenBank reference sequence (NC_030215), indicating a very high match with Rhina ancylostomus. Phylogenetic analysis shows that both samples cluster in a single clade with the reference sequence for Rhina ancylostomus, thereby confirming the molecular identification results. The species Rhina ancylostomus is listed as Critically Endangered (CR) on the IUCN Red List and is included in CITES Appendix II, meaning its use and trade are regulated internationally. At the national level, based on the Ministry of Marine Affairs and Fisheries Decree No. 66 of 2025 (KKP, 2025), this species is not included among protected fish species and is not listed under either full or limited protection.
Peningkatan Nilai Tambah Produk Perikanan Melalui Produksi Abon Ikan bagi Masyarakat Pulau Gili Iyang, Kabupaten Sumenep Chandra Trisna Pangestu; Sapto Andriyono; Esti Yunitasari; Tiyas Kusumaningrum; Praba Diyam Rachmawati; Nuzul Qur'aniati
PRAXIS: Jurnal Pengabdian kepada Masyarakat Vol. 5 No. 1 (2026): PRAXIS: Jurnal Pengabdian kepada Masyarakat (September)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47776/praxis.v5i1.07

Abstract

This community service program aims to enhance the added value of fishery products through the production of fish floss (abon ikan) in Gili Iyang Island, Sumenep Regency. The program focuses on empowering the local community by providing skills in processing fish into high-value economic products. Fish floss was chosen due to its high protein and omega-3 fatty acid content, which offers health benefits and has a long shelf life, making it suitable for areas with abundant fish production but limited development of derivative products. The community service activities were carried out in three main stages: (1) surveys and discussions with community leaders to understand local conditions and needs, (2) training in fish floss production and packaging using demonstration and simulation methods to ensure effective skill transfer, and (3) education on the nutritional value and health benefits of consuming processed fish products. The training also included simple packaging techniques, such as vacuum sealing, to improve product quality and shelf life. The outcomes of this program have enhanced the community's ability to process fish into value-added products, strengthened food security, and created new business opportunities. Furthermore, it is expected that the program will encourage changes in consumption patterns, promoting greater use of local fishery products. With these skills, the people of Gili Iyang Island can improve their economy through the development of small- and medium-scale fish processing businesses.  
Co-Authors Esti Yunitasari Heru Pramono Nuzul Qur'aniati Praba Diyam Rachmawati Sapto Andriyono Sapto Andriyono Tiyas Kusumaningrum