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Septiana Dwiyanti
Aquaculture Study Program, Department of Fisheries and Marine Science, Faculty of Agriculture, University of Mataram

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Application of Commercial Probiotics to Control Vibrio sp. in Whiteleg Shrimp (Litopenaeus vannamei) Culture at PT Sumber Agromina Jaya Raudatul Auliya; Septiana Dwiyanti
Journal of Fish Health Vol. 6 No. 2 (2026): Journal of Fish Health
Publisher : Study Program of Aquaculture, Department of Fisheries and Marine Science, Faculty of Agriculture, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v6i2.9937

Abstract

Whiteleg shrimp (Litopenaeus vannamei) is a leading aquaculture commodity with high economic value and can be farmed intensively with high stocking density. Intensive farming systems have the potential to increase the accumulation of organic matter and metabolic waste, which can trigger the growth of pathogenic bacteria such as Vibrio sp. The presence of these bacteria can cause health disturbances in shrimp and reduce survival rates. The purpose of this study is to determine and test the effectiveness of probiotics in suppressing the population of Vibrio sp. in whiteleg shrimp (Litopenaeus vannamei) farming. Data collection methods were carried out primarily and secondarily through direct observation, interviews, active participation in the field, documentation, and literature studies. Control techniques were performed through several stages, namely pond preparation, probiotic culture using molasses and skim milk, mixing probiotics into feed, application of probiotics to ponds. as well as routine monitoring of water quality. The observed parameters include nitrate (NO₃), nitrite (NO₂), ammonium (NH₄), phosphate (PO₄), alkalinity, Total Organic Matter (TOM), and the total population of Vibrio sp. The results of the study showed that the application of commercial probiotics was effective in suppressing the growth of Vibrio sp. with a population density of 103-104 CFU/ml during cultivation through the mechanisms of nutrient competition and the production of antibacterial compounds, as well as helping to maintain the stability of pond water quality within the optimal range for shrimp growth.
The Effectiveness of Gracilaria sp. as a Biofilter in Reducing Aquaculture Waste Based on Planting Location in the Waters of Ujung Betok, East Lombok Septiana Dwiyanti; Bagus Dwi Hari Setyono; Awan Dermawan; Muhammad Sumsanto; Muhammad Marzuki; Arman Ainul Ramdani
Journal of Fish Health Vol. 6 No. 3 (2026): Journal of Fish Health
Publisher : Study Program of Aquaculture, Department of Fisheries and Marine Science, Faculty of Agriculture, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v6i3.11857

Abstract

Aquaculture waste can increase dissolved inorganic nutrient concentrations, making seaweed-based treatment an important component of integrated biological nutrient management. This study aimed to evaluate strategic planting locations of Gracilaria sp. around floating net cages and assess its effectiveness as a natural biofilter for reducing nitrogenous waste in Ujung Betok waters, East Lombok. A Completely Randomized Design (CRD) was applied with three planting-distance treatments and three replicates. Total Ammonia Nitrogen (TAN) concentrations ranged from 0.29–0.31 mg/L at the beginning and 0.24–0.29 mg/L at the end of the 30-day culture period. ANOVA showed no significant difference in initial TAN among treatments (p = 0.868), whereas final TAN differed significantly among planting locations (p = 0.001). Final total nitrogen content in Gracilaria sp. tissue ranged from 0.287–0.330%, with the highest value in T2 (0.330 ± 0.050%), followed by T3 (0.303 ± 0.006%) and T1 (0.287 ± 0.015%). Planting location did not significantly affect tissue total nitrogen content (p > 0.05). Tissue nitrogen reduction was 13.03% in T1, 0.00% in T2, and 8.18% in T3. TAN reduction was highest in T1, decreasing from 0.31 to 0.24 mg/L or 22.58%, followed by T2 at 3.33%, while T3 showed no reduction. These results indicate that Gracilaria sp. can contribute to nitrogen removal, with the 25 m planting location showing the best biofiltration performance.