Mas Alvy Dwi Sayaza
Department of Soil Science and Land Resources, Faculty of Agriculture, IPB University

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Isolation and Characterization of Nutrient-Providing Microbes from Waste Soil and Grass Soil for Bio-Organic Fertilizer Formulation Mas Alvy Dwi Sayaza; Ilma Nadia Wardani; Abner Atmajendra Waluyaning Jati; Alya Wydia Utami; Ram Muhammad Zidan; Izzah Zen Salsabila Putri; Muhammad Amin Nur Zamzamy; Rima Sri Wahyuni
Jurnal Rekayasa Lingkungan dan Biosistem Vol. 3 No. 2 (2025): Jurnal Relibi Vol.3 No.2 2025
Publisher : Universitas Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/relibi.v3i2.2336

Abstract

Intensive agricultural practices have degraded soil fertility, increasing dependence on chemical fertilizers. This study aimed to isolate and characterize nutrient-providing microbes from waste soil and grass soil at IPB University and formulate them into bio-organic fertilizer. Rhizobium was isolated from peanut root nodules using YEMA medium, potassium solubilizing microbes (KSM) from grass soil using Alexandrov medium, and phosphate solubilizing microbes (PSM) from waste soil using Pikovskaya medium. Results showed Rhizobium population of 700 CFU with typical morphology. KSM population reached 1,036,333 CFU/g soil, while PSM reached 1,255,833 CFU/g soil. Both KSM and PSM demonstrated positive solubilization activity with indices of 2.34–2.66. Antagonism tests confirmed compatibility among the three isolates. Bio-organic fertilizer was successfully formulated by combining compost, molasses (5%), and the three microbial isolates in pellet form. Indigenous microbes from different soil ecosystems show promising potential as biofertilizer agents providing balanced N, P, and K nutrients through sustainable biological mechanisms.
Soil Penetration Resistance in Relation to Depth, Geomorphic Position and Moisture in Wonosari Village, Kendal Regency Mas Alvy Dwi Sayaza; Abner Atmajendra Waluyaning Jati
Jurnal Rekayasa Lingkungan dan Biosistem Vol. 4 No. 1 (2026): Jurnal Relibi Vol.4 No.1 2026
Publisher : Universitas Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/relibi.v4i1.3073

Abstract

Soil compaction strongly affects root growth, water infiltration and agricultural productivity. This study analysed the effect of soil depth and geomorphic position on soil penetration resistance, the standard field proxy for compaction, and its relationship with soil moisture in Wonosari Village, Kendal Regency, Central Java. Measurements were made in June 2026 at 47 sampling points using a hand-operated cone penetrometer at four depth intervals (0–10, 10–20, 20–30 and 30–40 cm), with three to four replications per point, together with land morphology classification and soil moisture measurement. Morphology could not be assigned at eight points, so morphology and moisture analyses used 39 points. Predominantly non-parametric methods were applied. Penetration resistance increased consistently with depth, from a mean of 804 kPa in the surface layer to 1,728 kPa at 30–40 cm, while variability was greatest at the surface. The convex/upper class recorded the highest resistance (1,630 kPa) and differed significantly from backslopes (1,112 kPa). No interaction between depth and morphology was detected, and no significant relationship with soil moisture was found, although statistical power was low in both cases. Because bulk density was not measured, conclusions about compaction remain inferential. Landscape position should be considered when preparing land for agroforestry development.