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All Journal Jurnal EduHealth Journal of Health and Nutrition Research
Kiki Sanjaya
Department of Environmental Health, Tadulako University, Indonesia
Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Environmental and Behavioral Factors Associated with the Presence of Aedes aegypti Larvae in the Sangurara Community Health Center Area, Palu City Widiana Widiana; Kiki Sanjaya; Adhe Sofyan Anas; Nur Azizah Azzahra; Alchamdani Alchamdani; Riri Suwahyuni Wahid
Jurnal EduHealth Vol. 17 No. 02 (2026): Jurnal EduHealt, April - June
Publisher : Sean Institute

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Abstract

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus transmitted through the bite of the Aedes aegypti mosquito. The presence of mosquito larvae in the household environment is an important indicator in assessing the risk of transmission of this disease. According to the World Health Organization (WHO), there are approximately 390 million dengue virus infections worldwide each year, with approximately 96 million cases showing clinical manifestations. In Indonesia, DHF is a public health problem that continues to be monitored. In the working area of the Sangurara Community Health Center in Palu City, cases were recorded at 79 in 2022, increasing to 96 in 2023, and decreasing to 70 in 2024. This study aims to determine the relationship between temperature, humidity, and knowledge with the presence of Aedes aegypti mosquito larvae. This study employed a quantitative cross-sectional design  using the Fisher's Exact Test and the Fisher Freeman Halton Exact Tes. The study population consisted of all household heads in the Sangurara Community Health Center working area totaling 13,591 families with a sample of 100 households. Data collection was carried out through examining larvae in all water containers in respondents’ homes and interviews using questionnaires. The results showed HI values of 20%, CI 23%, BI 40%, and ABJ 80% indicating a high larval density category. Bivariate analysis showed a significant relationship between temperature ( p = 0.016), humidity ( p = 0.039), and knowledge ( p = 0.012) with the presence of Aedes aegypti mosquito larvae. Knowledge was identified as the most influential factor associated with the presence of larvae. The conclusion shows that environmental factors (temperature and humidity) and knowledge are related to the presence of larvae so that it is necessary to strengthen larva surveillance and increase Mosquito Nest Eradication (PSN) practices by the community to reduce larva density and the risk of dengue transmission.
Ecoenzymes as Biocatalysts: Accelerating Biofilm Formation in Wastewater Biofilters Pitriani Pitriani; Kiki Sanjaya; Alchamdani Alchamdani; Ade Sofyan Anas; Riri Suwahyuni Wahid; Sendhy Krisna Sari
Journal of Health and Nutrition Research Vol. 5 No. 2 (2026)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jhnresearch.v5i2.1125

Abstract

Biofilters have advantages in waste treatment, but limitations related to the formation time of biofilms, which play an important role in reducing pollutants in wastewater. The addition of biocatalysts such as ecoenzymes can be an innovative solution in accelerating biofilm formation. This study aimed to examine the effectiveness of adding ecoenzyme to biofilter in accelerating biofilm formation. This research is quantitative with an experimental approach using a completely randomized design (CRD), The test was conducted by adding 10% orange peel ecoenzyme, where the characteristic test shows that this ecoenzyme is better quality than those made from vegetable waste. Data collection was carried out through: (1) Field observation, (2) In-situ measurement, and (3) Microscopic observation. The data was analyzed descriptively to examine the quality of ecoenzymes, meanwhile, biofilm development was observed microscopically. The result found the ecoenzyme has a distinctive fermented aroma, is dark brown colour, pH < 4 and normal temperature, this indicates that the ecoenzyme is of good quality.  Identification of lactic acid bacteria (LAB) revealed 53 colonies with large and small round morphology and a cloudy white colour. Lactobacillus bacteria dominated, both Gram-positive and Gram-negative found, rod-shaped, and large round colonies. Based on microscopic observation, biofilm formation in the control group was slower than in the samples treated with 10% ecoenzyme, a biofilm formation began to appear on the first day. While in the control samples it only appeared on the third day. This indicates that the ecoenzyme addition may accelerate biofilm formation.
Co-Authors Ade Sofyan Anas Adhe Sofyan Anas Alchamdani Alchamdani Nur Azizah Azzahra Pitriani Pitriani Riri Suwahyuni Wahid Sendhy Krisna Sari Widiana Widiana