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Ani Lestari
Program Studi Agroteknologi Fakultas Pertanian, Universitas Singaperbangsa, Karawang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Aplikasi Asam Humat dan Pupuk Organik Cair Ekstrak Rumput Laut untuk Meningkatkan Pertumbuhan dan Kandungan Flavonoid Brassica juncea L. Tia Setiawati; Sania Septiani Fitria; Mohamad Nurzaman; Rusdi Hasan; Asep Zainal Mutaqin; Ani Lestari
Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v11i2.5755

Abstract

This research was conducted with the aim of obtaining the optimal dose of humic acid (HA) and liquid organic fertilizer (LOF) from seaweed extract that can enhance the growth and total flavonoid content of Brassica juncea L. The method used in this study was experimental with a randomized complete block design with two factors. The first factor was HA, consisting of 4 dose levels: 0, 4, 8, and 12 g/kg, while the second factor was LOF from seaweed extract, consisting of 4 dose levels: 0, 1, 2, and 3 mL/L. Each treatment was replicated four times. Parameters were observed on the 42nd day, including plant height, leaf number, leaf area, dry weight, and total flavonoids. Data were analyzed using Analysis of Variance (ANOVA) and followed by Duncan's Multiple Range Test with a confidence level of 95%. The results showed that the dose of 4 g/kg HA and 2 mL/L LOF was the best dose for enhancing growth and total flavonoid content, producing an average plant height of 19.25 cm, a leaf number of 5.75, a total leaf area of 179.8 cm2, a dry weight of 0.57 grams, and a total flavonoid content of 0.106 mg QE/g sample.
Karakteristik Kalus dari Eksplan Batang Planlet Krisan (Chrysanthemum morifolium Ramat) pada Media dengan Konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan 6-Benzylaminopurine (BAP) serta Kondisi Pencahayaan Tia Setiawati; Annisa Nur Arofah; Ani Lestari; Rusdi Hasan
Pro-Life Vol. 11 No. 3 (2024): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v11i3.6404

Abstract

This research aimed to obtain the optimal concentration combination of 2,4-D and BAP growth regulators for inducing callus from the stem explant of Chrysanthemum (C. morifolium Ramat) in bright and dark conditions. Stem explants of chrysanthemum were grown in culture medium supplemented with various concentrations and combinations of 2,4-D and BAP under different lighting conditions for 45 days after culture. A laboratory-scale experimental method was used in this research using Completely Randomized Design (CRD). The observed parameters were induction time, percentage formation, size, fresh weight, dry weight, texture, color, and various respones generated by callus. All data were analyzed descriptively. The results showed that all concentrations and combinations of growth regulators could induce callus. In bright condition, the fastest callus induction time was 7 days after culture; most calli were dark green and dark brown colored with compact texture; the callus size was 1.36 cm; also, the highest dry weight generated by callus was 0.17 gram. Meanwhile, in dark conditions, the fastest callus induction time was 6 days after culture; most calli were light green and light brown colored with compact texture; the callus size was 1.18 cm; and the highest dry weight generated by callus was 0.15 gram.
Co-Authors Annisa Nur Arofah Asep Zainal Mutaqin Mohamad Nurzaman Rusdi Hasan Sania Septiani Fitria Tia Setiawati