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All Journal Klinikal Sains (Jurnal Analis Kesehatan)
Akhmad Mubarok
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology

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PERBANDINGAN PERTUMBUHAN Aspergillus niger PADA MEDIA POTATO DEXTROSE AGAR (PDA) DAN MEDIA ALTERNATIF DARI SINGKONG, UBI JALAR, DAN BEKATUL Indah Lestari; Akhmad Mubarok; Imam Agus Faizal
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4955

Abstract

Aspergillus niger is one of the fungi that can produce citric acid and is often used in some studies because of its resistance to very low acidic conditions. Potato Dextrose Agar medium is a common medium for culturing fungi in the laboratory. Alternative media are media that use materials from nature whose content is not yet known in detail. Rubber cassava has a carbohydrate content of 98.4674%. The carbohydrate content of white sweet potato is 28.78%. Rice bran has a carbohydrate content of 84.36%. The purpose of this study was to determine the comparison of the growth diameter of Aspergillus niger fungi on Potato Dextrose Agar (PDA) media and alternative media made from cassava, sweet potato, and rice bran. The research used a pure experimental method. Aspergillus niger isolates were observed microscopically, after which they were planted on PDA media and alternative media, incubated at 37°C for 72 hours, then the diameter of fungal growth was measured. The results showed that the alternative media that had the largest Aspergillus niger fungal growth diameter compared to PDA media as a control was alternative media from rice bran with the smallest diameter of 40 mm and the largest diameter of 80 mm.
RESISTENSI ANTIBIOTIK DAN PERTUMBUHAN STAPHYLOCOCCUS AUREUS PADA MEDIA AGAR DARAH Aldinova Gondo Ruming Asmoro; Yusuf Eko Nugroho; Akhmad Mubarok
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5283

Abstract

A microorganism growth medium is a material containing nutrients that support the growth and development of microorganisms. This nutrient is called the culture medium, and the growth process is known as culture, which can be enriched by the addition of blood. The aim of the researchers was to examine human blood, sheep's blood and cow's blood as growth media for Staphylococcus aureus bacteria. Is there a difference in the resistance test and growth patterns of Staplococcous aureus bacteria in human blood, sheep's blood and cow's blood media. Growth media in human blood, sheep's blood and cow's blood media. Bacterial growth in human blood media with round-shaped colonies, yellowish white with convex elevation, smooth surface, and medium size on cattle blood with a different type of hemolysis with negative results. smooth, and medium sized on sheep blood media with different types of hemolysis with positive results. Bacterial growth on cow blood media bacterial growth on cow blood media colonies are round, yellowish white with convex elevation, smooth surface, and medium sized on cow blood with hemolysis type which is different from a negative result. conclusion is that the Stapylococcus aureus bacteria has the same inhibitory zone in human blood, sheep blood and cow blood media. There is no difference between human blood, sheep blood and cow blood media. The difference in the type of hemolysis in sheep blood is because it has beta hemolysis type and in human blood, and cow's blood has gamma hemolysis.. Key words: Staphylococcous aureus, growth patterns, blood agar media
Co-Authors Aldinova Gondo Ruming Asmoro Imam Agus Faizal Indah Lestari Yusuf Eko Nugroho