Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Pertanian Indonesia
Titi Candra Sunarti
Department of Agricultural Industrial Technology, Faculty of Agricultural Technology, IPB University, IPB Campus Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Selection of Proteolytic Lactic Acid Bacteria with Probiotic Properties for Fish Protein Hydrolyzate Production Deford Cristy Birahy; Titi Candra Sunarti; Anja Meryandini
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.31

Abstract

This study aimed to select a proteolytic LAB with probiotic properties that can be applied to manufacture protein hydrolysate from fish heads. Tests on 20 isolates of LAB showed that nine isolates were proteolytic and non-pathogenic. A total of 5 isolates could grow well at 0.5% bile salt stress, and 3 of them could grow at pH 3. These three isolates had antagonistic ability against Salmonella bacteria, and one isolate was sensitive to the antibiotic tested. Molecular identification of the selected LAB isolates showed a 100% sequence similarity with Pediococcus pentosaceus with accession number MT515895.1. The LAB isolate has high proteolytic activity since it can increase the soluble fraction of fish meal powder from 32.10% to 88.38% in 48 hr. Production of protein hydrolysate using tuna waste was carried out for 30 days. Tuna waste protein hydrolysate had a medium antioxidant activity of 25.57 ± 0.93%. The hydrolyzed protein comprised 17 amino acids, including nine non-essential and eight essential amino acids, and is dominated by glutamic acid. Selected LAB isolate is potentially used in protein hydrolysate production, especially for flavor enhancers. Keywords: antagonistic, amino acid, Pediococcus pentosaceus
Co-Authors Anja Meryandini Deford Cristy Birahy