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All Journal SRIWIJAYA JOURNAL OF MEDICINE International Journal of Psychology and Health Science
Eny Yulianti
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta, Indonesia
Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Phytochemical Profile and Antioxidant Activity of Green Onion (Allium fistulosum L.) Extract Lis Diana Husni Putri; David Limanan; Eny Yulianti; Frans Ferdinal
International Journal of Psychology and Health Science Vol. 4 No. 3 (2026): International Journal of Psychology and Health Science (July - September 2026)
Publisher : Greenation Publisher & Yayasan Global Research National

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/ijphs.v4i3.2199

Abstract

Oxidative stress plays a pivotal role in the progression of various degenerative diseases due to excessive production of reactive oxygen species (ROS) that overwhelms the body's antioxidant defense system. Green onion (Allium fistulosum L.) is an herbal plant known to contain diverse bioactive compounds with potential as natural antioxidants. This study aimed to analyze the phytochemical profile and antioxidant activity of green onion extract in vitro. Extraction was performed using the percolation method with methanol. Qualitative phytochemical analysis was conducted using specific reagents. Antioxidant capacity was subsequently evaluated using DPPH, ABTS, and FRAP assays, each referenced against Trolox as the standard compound. Results showed that the extract had a yield of 20.36% and contained alkaloids, betacyanins, cardiac glycosides, glycosides, coumarins, flavonoids, phenols, quinones, saponins, steroids, terpenoids, and tannins. Antioxidant activity yielded IC₅₀ values of 185.86 µg/mL (DPPH), 91.60 µg/mL (ABTS), and 15.39 µg/mL (FRAP). These findings indicate that green onion extract possesses considerable potential as a natural antioxidant source for further development.
Penta Herbs Forte: The Effect of PHF Extract on Superoxide Dismutase Activity in Hypoxic Sprague Dawley Rat’s Heart Jessie Farica Gunardi; Noer Saelan Tadjudin; Frans Ferdinal; David Limanan; Eny Yulianti
Sriwijaya Journal of Medicine Vol. 9 No. 1 (2026): Vol 9, No 1, 2026
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v9i1.382

Abstract

Hypoxia is a condition in which tissues receive insufficient oxygen, leading to increased production of reactive oxygen species (ROS) and triggering oxidative stress that can cause heart damage. Penta Herbs Forte (PHF), a combination of Blumea balsamifera, Curcuma xanthorrhiza, Zingiber officinale var. rubrum, Phyllanthus niruri, and Andrographis paniculata, contains bioactive compounds with antioxidant properties that may help strengthen the body’s defense against oxidative injury. This study aimed to evaluate the effect of PHF extract on the activity of the antioxidant enzyme Superoxide Dismutase (SOD) in the hearts of hypoxia-induced Sprague Dawley rats. The research included in vitro assessments (total phenolic content, BSLT toxicity, and FRAP assays) and in vivo experiments involving eight groups of rats receiving PHF extract with different hypoxia exposure durations (0, 1, 7, and 14 days). Heart tissues were processed into homogenates, and SOD activity was measure by spectrophotometry, followed by statistical analysis using the Mann-Whitney and Kruskal-Wallis tests. Although PHF groups showed higher SOD activity, significant differences between control and treated groups were observed only at 1-day hypoxia. These findings suggest that PHF may help protect the heart from hypoxia-related oxidative stress by enhancing antioxidant enzyme activity.
Co-Authors David Limanan Frans Ferdinal Jessie Farica Gunardi Lis Diana Husni Putri Noer Saelan Tadjudin