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All Journal Bioscientist : Jurnal Ilmiah Biologi
Kurnia Dwi Agustin
Universitas Negeri Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Microbiological Quality and Presumptive Enterobacter Contamination in Beef Liver Sold at Traditional Markets in Sidoarjo City Kurnia Dwi Agustin; Isnawati
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20732

Abstract

This study aimed to evaluate the microbiological quality of beef liver sold in traditional markets in Sidoarjo City by determining the total plate count (TPC) of total microorganisms and Enterobacteriaceae, as well as conducting presumptive identification of the genus Enterobacter through biochemical testing. The TPC results for total microorganisms showed that 7 of the 10 samples exceeded the maximum limit specified by the Indonesian National Standard (SNI). In addition, all 10 samples exceeded the Enterobacteriaceae limit established in SNI 9159:2023. Biochemical test results indicated that isolate B2, obtained from Porong Market, exhibited characteristics similar to those of Enterobacter cloacae, namely indole (–), H₂S (–), motility (+), methyl red (MR)    (–), Voges–Proskauer (VP) (+), citrate (+), and urease (+). The high levels of microbial contamination indicate that beef liver samples from traditional markets in Sidoarjo City did not meet the microbiological standards for safe consumption. Therefore, to obtain its nutritional benefits while minimizing the risk of microbial infection, beef liver should not be consumed raw and should be cooked thoroughly at approximately 70°C.
Co-Authors Isnawati