Article Per Year (5 Year)
p-Index From 2021 - 2026
0.408
P-Index
This Author published in this journals
All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Kristamtini Kristamtini
Research Center for Food Crops, National Research and Innovation Agency, Bogor 16915
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
MOLECULAR ANALYSIS OF WAXY GENE MARKERS IN SORGHUM CROSSES KD4 AND BONTEB GUNUNGKIDUL Arif Muazam; Kristamtini Kristamtini; Setyorini Widyayanti; Yudhistira Nugraha; Rina Sri Kasiamdari; Budi Setiadi Daryono
BIOTROPIA Vol. 32 No. 3 (2025): BIOTROPIA Vol. 32 No. 3 December 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.3.2457

Abstract

HIGHLIGHTS- Local sorghum crosses show unique fixation of the waxy starch gene allele- Only Wxc allele is expressed, while other waxy alleles are not detected- Waxy allele expression strongly relates to low amylose grain quality- Marker-based selection supports breeding of soft-textured sorghum- Findings enhance sorghum use for food, feed, and industrial applicationsABSTRACTSorghum (Sorghum bicolor L.) is a food crop exhibiting resilience to extreme environmental conditions and potential to develop as an alternative food source. Quality of sorghum seeds is significantly influenced by starch composition in endosperm regulated by waxy (Wx) gene. This gene has several major alleles: Wxa, Wxb, and Wxc, playing roles in amylopectin and amylose syntheses. The purpose of study is to analyze the expression of Wx alleles in the crossbreeding of sorghum cultivars KD4 and Bonteb Gunungkidul. Main method used was molecular marker-based PCR method. Leaf and seed samples of sorghum were collected and extracted using the CTAB method to obtain high-quality genomic DNA. PCR reactions were performed with specific primers for each allele, and the amplification results were analyzed through 1.5% agarose gel electrophoresis. Statistical analysis was performed to ensure result significance through: Chi-Square Test to determine relationships between waxy allele expression with genetic segregation within cross populations, Allele Frequency Analysis to determine distribution of waxy genotypes within populations by comparing counts showing expressions of Wxa, Wxb, and Wxc, and Pearson Correlation Test to evaluate relationships between waxy gene expression with specific agronomic traits (e.g., amylose content). Main findings showed that only Wxc allele did exhibit a clear amplification band, while Wxa and Wxb did not show significant expression. Further studies are needed to understand the regulation of Wx gene expression and its potential implications to molecular selection, ultimately enhancing sorghum quality for both food and industrial applications.
PROXIMATE, TANNIN, AND AMYLOSE IN SIX SORGHUM VARIETIES ACROSS TWO PLANTING SEASONS Arif Muazam; Kristamtini Kristamtini; Setyorini Widyayanti; Yudhistira Nugraha; Rina Sri Kasiamdari; Budi Setiadi Daryono
BIOTROPIA Vol. 32 No. 2 (2025): BIOTROPIA Vol. 32 No. 2 August 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.2.2461

Abstract

ARTICLE HIGLIGHTS- Sorghum nutrition varies across varieties and growing seasons- Dry season increases starch and amylose content in some varieties- Rainy season enhances protein levels in sorghum grains- Genotype-environment interaction affects sorghum nutritional quality- Sorghum adaptation supports food diversification and climate resilienceABSTRACTSorghum (Sorghum bicolor (L.) Moench) is a potential staple food in Indonesia, offering an alternative to rice and corn due to its adaptability to marginal lands and drought tolerance. However, research on the impact of environmental factors, particularly different growing seasons, to the nutritional composition of sorghum varieties are still lacking. This study aimed to analyze the proximate contents of tannin and amylose in six sorghum varieties cultivated in two different planting seasons (wet and dry seasons). A Completely Randomized Design with three replications was applied in this experiment. The tested varieties included three newly developed superior varieties (Bioguma, Samurai, and Kawali) and three local varieties (Black Bonteb, Red Glutinous Sorghum, and KD4). The results showed that proximate composition varied across varieties and seasons. Starch content was generally higher in the dry season, with the highest observed in Kawali (64.16%) and Bioguma (63.11%), while the lowest was in Black Wareng (11.99%). Similarly, protein content peaked in Black Wareng (7.68%) and Red Glutinous Sorghum (7.37%), but was the lowest in Samurai (4.48%). Tannin levels also fluctuated by season, where Bioguma, KD4, and Kawali exhibited higher tannin content in the dry season, whereas Samurai, Red Glutinous Sorghum, and Black Bonteb had lower tannin levels. Notably, amylose content was significantly higher in Red Glutinous Sorghum (4.72%) and Black Bonteb (4.62%) across both seasons, contributing to their fluffier texture when cooked. These findings highlight the influence of environmental conditions on sorghum nutritional quality, reinforcing its potential for food diversification, including processed sorghum flour as a substitute for wheat flour.
Co-Authors Arif Muazam Arif Muazam Budi Setiadi Daryono Budi Setiadi Daryono Rina Sri Kasiamdari Rina Sri Kasiamdari Setyorini Widyayanti Yudhistira Nugraha