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All Journal Panthera : Jurnal Ilmiah Pendidikan Sains dan Terapan
Eny Widhia Agustin
Program Studi Pendidikan Tata Kecantikan, Fakultas Teknik, Universitas Negeri Semarang, Jalan Kolonel H. R. Hadijanto, Semarang, Jawa Tengah 50229, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Mathematics Physics Social Sciences

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Uji Aktivitas Antioksidan Sediaan Face Mist Ekstrak Daun Pepaya California (Carica papaya L.) dengan Metode DPPH Jihan Zulfa Nabila; Eny Widhia Agustin
Panthera : Jurnal Ilmiah Pendidikan Sains dan Terapan Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/panthera.v6i2.1274

Abstract

Free radicals are a major cause of skin damage that can accelerate premature aging; therefore, the use of antioxidants, particularly from natural sources such as papaya leaves (Carica papaya L.), is essential due to their flavonoid, tannin, and polyphenol content. The application of antioxidants in a face mist formulation is considered practical as it is easy to use and provides a refreshing effect on the skin. This study aimed to evaluate the antioxidant activity of a face mist containing California papaya leaf extract using the DPPH method and to assess its physical characteristics. A quantitative experimental method with a post-test only design was employed using two formulations with different extract concentrations. The results showed that the IC₅₀ values of Formula I and Formula II were 2475.23 ppm and 812.14 ppm, respectively, indicating very weak antioxidant activity, although Formula II exhibited better performance. Physical evaluation revealed that both formulations were organoleptically stable and homogeneous; however, the pH values were below the acceptable skin range and the viscosity of Formula II was relatively high. In conclusion, the formulation demonstrates potential as a natural antioxidant product, but further optimization is required to improve its effectiveness and quality.
Kelayakan Ekstrak Daun Jambu Biji (Psidium guajava L.) sebagai Micellar Water dalam Menghambat Propionibacterium acnes Keysha Alea Chairani; Eny Widhia Agustin
Panthera : Jurnal Ilmiah Pendidikan Sains dan Terapan Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/panthera.v6i2.1300

Abstract

Acne (Acne vulgaris) is a skin problem often caused by Propionibacterium acnes bacterial infection. Guava leaves (Psidium guajava L.) contain antibacterial compounds, but their use as facial cleansing cosmetic preparations in the form of micellar water has not been widely done. This experimental study aims to determine the feasibility of guava leaf extract micellar water preparations in inhibiting the growth of Propionibacterium acnes. The preparations were formulated into two variations of extract concentration, namely F1 (50%) and F2 (100%). The evaluation stage of the preparations included organoleptic tests, homogeneity, pH, viscosity, and antibacterial activity testing using the well method. The results of physical tests showed that both formulas were liquid in texture, dark brown in color, and had a distinctive odor of guava leaves. However, the preparations were proven to be inhomogeneous due to the presence of particle deposits. The average pH value of the preparations was classified as acidic and was below the physiological standard of the skin (<4.5) which was 3.05 for F1 and 3.19 for F2. Antibacterial testing showed weak inhibition (<5 mm), with an average F1 inhibition zone of 0.961 ± 0.0300 mm and F2 of 1.142 ± 0.0226 mm. One-way ANOVA analysis showed a statistically significant difference between the treatment groups (p<0.05). However, the preparation was not yet clinically effective, thus concluding that guava leaf extract micellar water is not yet suitable for optimal application due to the physical instability triggered by the high extract concentration and its inadequate ability to penetrate the complex cell wall of Propionibacterium acnes. Further research is needed to optimize the formulation of this preparation.
Co-Authors Jihan Zulfa Nabila Keysha Alea Chairani