Putri Indah Ningtias
Cipelang Livestock Embryo Center, Bogor, Indonesia

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Evaluation of Superovulation Results on in Vivo Embryo Production in Three Different Cows Breeds at The Livestock Embryo Center Rismasari; Dwi Wijayanti; Fahrudin Darlian; Putri Indah Ningtias; Andri Kusmayadi
Jurnal Peternakan Nusantara Vol. 12 No. 1 (2026)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jpn.v12i1.18415

Abstract

The purpose of this study was to compare the superovulation response in several breeds, namely Angus, Friesian Holstein (FH), and Ongole crossbreed (OC). A total of 152 cows were used in this study, namely 39 Angus cows, 62 Friesian Holstein cows, and 51 Ongole crossbreed cows. The parameters of this study were the number of CL, response rate, recovery rate, number of embryos collected (1, 2, 3, 4 (DG/UFO)), proportion of transferable embryos, proportion of DG, and proportion of UFO. The collected data were tested using the Kruskall-Wallis test, if there was a difference, it was continued using the Mann-Whitney test. The results of this study included the number of CL, recovery rate, total embryos collected, proportion of DG, and proportion of UFO of Angus, FH, and OC cows showed significantly different results (P <0.05), the response rate showed very significantly different results (P <0.01), but the proportion of transferable embryos was not significantly different (P > 0.05). This study concludes that CO cattle have the highest average of CL, response rate, recovery rate, and total embryos collected. Meanwhile, Angus cattle produce the lowest percentage of DG and UFO embryos
Comparative Superovulatory Response, Embryo Recovery, and Embryo Quality in Holstein-Friesian and Limousin Donor Cattle in Indonesia Anggita Dian Pramudhita; Detry Al Bukhary; Putri Utami; Habib Asshidiq Syah; Anny Rosmayanti; Putri Indah Ningtias; Fahrudin Darlian; Trinil Susilawati; Aulia Puspita Anugra Yekti
ANIMAL PRODUCTION Vol. 28 No. 1 (2026)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2026.28.1.486

Abstract

Embryo transfer (ET) is a reproductive biotechnology used to accelerate the dissemination of superior genetics. However, its success under Indonesian tropical conditions remains variable and may be influenced by physiological and adaptive differences between dairy and beef cattle used as embryo donors. This study compared in vivo embryo production between Holstein-Friesian (HF) and Limousin donor cattle. The study was designed as a comparative observational study using selected donor cattle, comprising 11 HF and 25 Limousin cows maintained under standardized ET management. The observed parameters included superovulatory response, represented by the number of corpora lutea (CL), total ova/embryos collected, fertile embryos, transferable embryos, embryo quality, response rate, and recovery rate. Data were analyzed using descriptive statistics, and breed differences were analyzed using Welch’s independent t-test when variance heterogeneity was detected, and Cohen’s d was used to estimate effect size. Limousin cows showed higher CL counts than HF cows (12.72 ± 6.98 vs. 6.55 ± 2.88; P < 0.001), produced more total ova/embryos (11.44 ± 6.45 vs. 6.00 ± 3.00; P < 0.001), fertile embryos (9.16 ± 5.17 vs. 3.45 ± 2.81; P < 0.001), and transferable embryos (5.76 ± 4.29 vs. 1.91 ± 2.07; P < 0.001). Response rate between HF and Limousin cows was 94.12% vs. 92.86%, as was the recovery rate (89.92% vs. 90.12%). Overall, these findings indicate that under the conditions of this study, Limousin cattle exhibited higher superovulatory responses and embryo production than HF cattle, although breed-specific protocol optimization remains necessary.