Nino Nasution
Universitas Sumatera Utara

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The Effect of Intralesional Propolis on Transforming Growth Factor-β (TGF-β) Expression in Tendon Healing: An Experimental Study in a Wistar Rat Model of Partial Achilles Tendon Rupture Luthfi Walikram; Nino Nasution; Andriandi Andriandi
Indonesian Journal of Global Health Research Vol. 8 No. 3 (2026): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v8i3.1524

Abstract

Transforming growth factor-beta (TGF-β) regulates tendon repair, but dysregulated expression contributes to fibrosis. Propolis contains flavonoids and phenolics with immunomodulatory activity and may influence TGF-β signaling. This study evaluated intralesional propolis on TGF-β expression in a Wistar rat partial Achilles rupture model. A true experimental post-test only control group design was used. Male Wistar rats underwent standardized partial Achilles transection (30–40% thickness) and were randomized to: 50% propolis 0.1 mL intralesional (n=9), 0.9% NaCl 0.1 mL (n=9), or no injection (n=9). On day 35, tendons were harvested for immunohistochemistry using an IHC World–based scoring (H-score 0–300) assessed in ≥5 high-power fields by two independent raters. Interobserver agreement was moderate (κ=0.444, p<0.001). TGF-β H-score categories differed significantly among groups (Kruskal–Wallis p<0.001). The propolis group shifted toward higher expression: 88.9% (8/9) were 101–200 and 11.1% (1/9) were 51–100, whereas both comparator groups clustered at 0–50 (44.4%) and 51–100 (55.6%), with none ≥101. Intralesional 50% propolis increased TGF-β immunohistochemical expression at day 35 after partial Achilles rupture, suggesting enhanced regenerative signaling during tendon healing. Further studies should assess dose–response effects and biomechanical outcomes in vivo.
Functional Outcomes after Temporary Kirschner-Wire Fixation Versus Early Latarjet Procedure in Old Unreduced Anterior Shoulder Dislocation: A Retrospective Cohort Study Nino Nasution; Pranajaya Dharma Kadar; Teuku Reza Syahputera
Indonesian Journal of Global Health Research Vol. 8 No. 6 (2026): Indonesian Journal of Global Health Research: December 2026
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v8i6.2765

Abstract

Old unreduced anterior shoulder dislocation (OUAD), defined as a dislocation unreduced for at least three weeks, is technically demanding because capsulolabral injury, glenoid bone loss, Hill-Sachs lesions, and capsular contracture may coexist. Open reduction with temporary K-wire fixation preserves the humeral head but does not reconstruct glenoid deficiency, while the Latarjet procedure provides an anterior bone block and sling effect. Objective to compare six-month functional outcomes after open reduction with temporary Kirschner-wire (K-wire) fixation and early Latarjet procedure in patients with old unreduced anterior shoulder dislocation (OUAD). This retrospective cohort included 34 adults with OUAD treated surgically between January 2022 and December 2025. Eighteen underwent open reduction with temporary K-wire fixation and 16 underwent early Latarjet stabilization. The primary outcome was the Constant-Murley Score (CMS) at six months. Baseline variables were compared using independent-samples tests or Fisher's exact test as appropriate; CMS subdomains were compared non-parametrically when distributional assumptions were not met. Mean age was 47.29 ± 16.96 years, and 21 patients (61.8%) were women. Dislocation duration was longer in the K-wire group than in the Latarjet group (19.83 ± 5.81 vs. 9.75 ± 6.10 weeks; P < 0.001), whereas glenoid bone loss >25% was more frequent in the Latarjet group (P = 0.003). Total CMS was comparable between groups (58.61 ± 11.09 vs. 60.93 ± 9.28; P = 0.515). The K-wire group had higher pain-domain scores, whereas the Latarjet group had higher activities-of-daily-living and range-of-motion scores. Motor strength was similar. At six months, temporary K-wire fixation and early Latarjet produced comparable total CMS in selected patients with OUAD, but the domain-level recovery profiles differed. These findings should be interpreted in light of baseline treatment-selection differences and the retrospective design.