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All Journal Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran
Gina Andyka Hutasoit
Departemen Patologi Anatomi, Fakultas Kedokteran, Universitas Tadulako
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Perbandingan PERBANDINGAN KUALITAS PREPARAT SITOPATOLOGI CAIRAN EFUSI PLEURA DENGAN PEWARNAAN GIEMSA DAN PAPANICOLAU Janita Aulogia Pamusok; Gina Andyka Hutasoit; Nur Syamsi; Rosa Dwi Wahyuni
Medika Alkhairaat: Jurnal Penelitian Kedokteran dan Kesehatan Vol 8 No 01 (2026): April
Publisher : Fakultas Kedokteran Universitas Alkhairaat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/ma.v8i01.424

Abstract

ABSTRAK Pemeriksaan sitopatologi berperan penting dalam menunjang diagnosis berbagai kondisi patologis, terutama pada kasus efusi pleura, dimana kualitas preparat sangat dipengaruhi oleh metode pewarnaan yang digunakan. Penelitian ini bertujuan untuk membandingkan kualitas preparat sitopatologi cairan efusi pleura menggunakan metode pewarnaan giemsa dan papanicolaou. Studi kuantitatif komparatif dengan desain post-test only dilakukan pada 52 sampel efusi pleura yang diperoleh melalui pengambilan sampel total dari pasien yang menjalani torakosentesis di Rumah Sakit Umum Undata Palu, pada Juli 2025. Setiap sampel dipreparasi menggunakan kedua teknik pewarnaan dan dievaluasi berdasarkan morfologi sel, kontras inti, kontras sitoplasma, dan kebersihan latar belakang. Uji Mann–Whitney U menunjukkan perbedaan signifikan antara kedua metode (p < 0,001), yang mengindikasikan bahwa pewarnaan papanicolaou memberikan kontras inti dan sitoplasma yang lebih unggul. ABSTRACT Cytopathological examination plays an important role in supporting the diagnosis of various pathological conditions, particularly in cases of pleural effusion, where the quality of the preparations is greatly influenced by the staining method used. This study aimed to compare the quality of cytopathological preparations of pleural effusion fluid using giemsa and papanicolaou staining methods. A comparative quantitative study with a post-test only design was conducted on 52 pleural effusion samples obtained through total sampling from patients undergoing thoracentesis at Undata General Hospital Palu, in July 2025. Each sample was prepared using both staining techniques and evaluated based on cell morphology, nuclear contrast, cytoplasmic contrast, and background cleanliness. The Mann–Whitney U test showed a significant difference between the two methods (p < 0.001), indicating that papanicolaou staining provides superior nuclear and cytoplasmic contrast.
THE EFFECT OF NBF (Neutral Buffered Formalin) 10% EARLY FIXATION ON UTERINE MESENCHYMAL CELL MORPHOLOGY IN A SIMPLE LABORATORY Mawar Rahmi; Gina Andyka Hutasoit; Junjun Fitriani; Amirah Basry
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran Vol. 11 No. 1 (2026): Maret
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/f5710y30

Abstract

An unoptimal duration of tissue fixation can cause degradation of cell morphology and reduce the accuracy of histopathological diagnosis. This study examined the effect of fixation duration using 10 percent Neutral Buffered Formalin on the morphology of uterine mesenchymal cells. The objective was to identify and compare their morphological characteristics after fixation for less than 2 weeks and more than 2 weeks and determine the optimal duration. A quasi-experimental design used 56 uterine leiomyoma samples divided into 2 fixation groups and processed with standard histopathology procedures using hematoxylin eosin staining. Morphological quality was evaluated based on nuclear color, cytoplasm, and cell membrane clarity using a 6-level assessment. Data were analyzed with descriptive statistics and a nonparametric comparative test. The group fixed for less than 2 weeks showed higher morphological values than the group fixed for more than 2 weeks, demonstrating clearer smooth muscle fibers, nuclei, and cytoplasm, while prolonged fixation produced shrunken nuclei and denser cytoplasm with reduced clarity. These findings indicate that fixation duration significantly influences the morphological quality of uterine mesenchymal cells, and fixation for less than 2 weeks yields superior morphology. This emphasizes the importance of appropriate fixation duration for producing reliable histopathological preparations.
Co-Authors Amirah Basry Janita Aulogia Pamusok Junjun Fitriani Mawar Rahmi Nur Syamsi Rosa Dwi Wahyuni