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All Journal Acta Biochimica Indonesiana
Anita Lidesna Shinta Amat
Department of Biomedical Sciences, Faculty of Medicine and Veterinary Medicine, Nusa Cendana University
Biochemistry, Genetics & Molecular Biology

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Dose-dependent nephroprotective effects of turmeric extract against diclofenac-induced kidney injury in rats Renya Rosari Amapiran; Anita Lidesna Shinta Amat; Desi Indriarini; Syeben HE Hietingwati; Tri Nugraheni
Acta Biochimica Indonesiana Vol. 8 No. 2 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.208

Abstract

Background: Non-steroidal anti-inflammatory drugs, particularly diclofenac sodium, are widely prescribed but can cause nephrotoxicity through oxidative stress mechanisms. Turmeric (Curcuma longa L.) contains curcumin and other bioactive compounds with potent antioxidant properties that may protect against drug-induced kidney damage. Objective: To evaluate the dose-dependent effects of turmeric ethanol extract on kidney histopathology in rats with established diclofenac sodium-induced nephrotoxicity. Methods: Twenty-eight male Sprague Dawley rats were randomly divided into four groups: normal control, diclofenac (10 mg/kg for 7 days), and two treatment groups receiving diclofenac followed by turmeric extract at 100 mg/kg or 200 mg/kg for 14 days. Kidney histopathology was assessed using the Arsad scoring system by a blinded pathologist. Results: Significant differences existed between groups (p < 0.001). The negative control exhibited severe kidney damage with hydropic degeneration, granular casts, and cellular casts (mean score: 2.78 ± 0.24). Treatment with 100 mg/kg showed partial improvement (1.65 ± 0.31), while 200 mg/kg demonstrated substantial improvement approaching normal histology (0.52 ± 0.18) with only minimal residual damage. Conclusion: Turmeric extract demonstrates significant dose-dependent nephroprotective effects against diclofenac-induced kidney damage, with 200 mg/kg providing superior protection, suggesting potential therapeutic applications in mitigating NSAID-induced nephrotoxicity.
A study on the hepatoprotective effect of turmeric (Curcuma longa) extract on the liver histopathology of albino rats induced by sodium diclofenac Grasia Intan Praskawati Hibur; Anita Lidesna Shinta Amat; Magdarita Riwu; Arley Sadra Telussa
Acta Biochimica Indonesiana Vol. 8 No. 1 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.209

Abstract

Background: Diclofenac sodium, a widely used nonsteroidal anti-inflammatory drug (NSAID), causes significant hepatotoxicity through oxidative stress mechanisms. Turmeric (Curcuma longa L.), rich in curcuminoid antioxidants, may offer hepatoprotection. Objective: To evaluate the hepatoprotective effects of turmeric extract against diclofenac sodium-induced liver injury in rats. Methods: Twenty-eight male Sprague Dawley rats were randomly divided into four groups (n=7): normal control, negative control (diclofenac sodium 10 mg/kg BW for 7 days), and two treatment groups receiving diclofenac sodium followed by turmeric extract at 100 or 200 mg/kg BW for 14 days. Liver histopathology was assessed using hematoxylin-eosin staining. Data were analyzed descriptively. Results: Turmeric extract attenuated hepatocellular damage in a dose-dependent manner. The 200 mg/kg BW dose completely prevented necrosis, demonstrating superior hepatoprotection compared to 100 mg/kg BW. Conclusion: Turmeric extract exerts hepatoprotective effects against diclofenac-induced liver injury through attenuation of histopathological damage.
Effect of turmeric (Curcuma longa L.) extract on gastric histopathology of diclofenac sodium-induced rats Melania Lie; Anita Lidesna Shinta Amat; Rahel Rara Woda; Kartini Lidia
Acta Biochimica Indonesiana Vol. 8 No. 1 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.210

Abstract

Background: Prolonged use of diclofenac sodium can cause gastric mucosal damage through cyclooxygenase inhibition and oxidative stress. Turmeric (Curcuma longa L.) contains curcumin and other bioactive compounds with potential gastroprotective properties. Objective: To determine the dose-dependent gastroprotective effect of turmeric extract against diclofenac sodium-induced gastric mucosal injury in rats. Methods: This experimental study used 28 male Sprague-Dawley rats randomly allocated into four groups (n=7): normal control, negative control (diclofenac 10 mg/kgBW for 7 days), and two treatment groups receiving turmeric extract at 100 mg/kgBW (P1) or 200 mg/kgBW (P2) for 14 days following diclofenac induction. Gastric tissues were evaluated histopathologically using a qualitative description. Results: The negative control group showed severe erosion and inflammatory infiltration. Both treatment groups demonstrated gastroprotective effects with minimal epithelial damage and no inflammatory changes. The 200 mg/kgBW dose showed superior protection compared to 100 mg/kgBW. Conclusion: Turmeric extract provides dose-dependent gastroprotection against diclofenac-induced gastric injury, with 200 mg/kgBW demonstrating superior efficacy.
Co-Authors Arley Sadra Telussa Desi Indriarini Grasia Intan Praskawati Hibur Kartini Lidia Magdarita Riwu Melania Lie Rahel Rara Woda Renya Rosari Amapiran Syeben HE Hietingwati Tri Nugraheni