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All Journal Bioscientist : Jurnal Ilmiah Biologi
Yurnaliza
Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Isolation and Morphological Characterization of Fungal Isolates from Leachate of Namo Bintang Landfill, North Sumatra Niken Simanjuntak; Erman Munir; Yurnaliza
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18277

Abstract

This study aims to isolate and characterize the morphological characteristics of fungal colonies obtained from leachate water at a landfill site. To obtain fungi from leachate water, the following steps were taken: Fungi were isolated using the pour plate method with Potato Dextrose Agar (PDA) medium; morphological characterization of fungi was performed macroscopically and microscopically. Macroscopically, by observing the shape, edges, texture, and color of the fungal colonies, and microscopically, by observing the shape of the hyphae and the presence of asexual spores. The results showed that seventeen fungal isolates were successfully isolated from the leachate of the Namo Bintang landfill with different morphologies. The macroscopic characteristics of the fungal colonies showed that 15 fungal isolates had round colonies, and the other two isolates (NKD 07 and NKT 14) had irregular colonies. The edges of the fungal colonies varied, ranging from flat, wavy, to serrated. The color of the fungal colonies was predominantly gray and green. The texture of the fungal colonies varied from powdery, hairy, smooth, to cotton-like. Microscopic characteristics of the fungi show that most fungal isolates have septate hyphae and varied conidia shapes, such as round, oval, crescent, and tubular. These findings indicate that the leachate from the Namo Bintang landfill contains diverse fungi with different colony morphological characteristics, making it possible to develop them as biological agents.
Qualitative Screening and Activity Assay of L-asparaginase-Producing Fungi from Tempeh Niva Tahyat; Yurnaliza; Liana Dwi Sri Hastuti
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18443

Abstract

This study aims to isolate and determine the types of fungi in tempeh that are capable of producing the enzyme L-asparaginase and how the enzyme activity is produced. The following steps were taken: Fungi were isolated using the pour plate method with Potato Dextrose Agar (PDA) medium; morphological characterization of fungi was performed macroscopically and microscopically.The ability to produce asparaginase was assessed qualitatively using modified liquid M9 medium and quantitative assay of L-asparaginase activity with the Nessler reagent. Potential fungi were identified based on identification books. The results of fungal isolation from tempeh yielded of 7 fungal isolates. Qualitative selection of fungal isolates from tempeh capable of producing L-asparaginase resulted in 4 fungi capable of producing the L-asparaginase enzyme, indicated by a pink color change in the medium. Quantitative assay was performed using an enzyme activity test .The results of quantitative selection showed that the highest activity of L-asparaginase enzyme was found in fungal isolate with the code TP 3, reaching 16,386 U/mL, which has been identified macroscopically and microscopically and is classified as belonging to the genus Aspergillus. These findings indicate that fungi from tempeh have the potential to produce the enzyme L-asparaginase.
Co-Authors Erman Munir Liana Dwi Sri Hastuti Niken Simanjuntak Niva Tahyat