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All Journal Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Research of Scientia Naturalis
Nike Triwahyuningsih
Institut Pertanian INTAN Yogyakarta
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Electrical & Electronics Engineering Engineering Health Professions Industrial & Manufacturing Engineering Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Transportation Veterinary

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ISOLATION AND CHARACTERIZATION OF NOVEL ANTIBIOTIC-PRODUCING ACTINOBACTERIA FROM THE RHIZOSPHERE OF INDONESIAN MANGROVE FORESTS Nike Triwahyuningsih; Chen Mei; Anna Schneider
Research of Scientia Naturalis Vol. 2 No. 5 (2025)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/scientia.v2i5.2567

Abstract

The rise of antimicrobial resistance is a global health crisis, demanding the urgent discovery of novel antibiotics. Indonesian mangrove forests, as a unique and underexplored ecosystem, represent a promising frontier for bioprospecting novel microorganisms. The plant rhizosphere, a zone of intense microbial activity, is particularly rich in actinobacteria, a phylum renowned for its prolific production of bioactive secondary metabolites. This research aimed to isolate and characterize novel antibiotic-producing actinobacteria from the rhizosphere of Indonesian mangrove plants. Rhizosphere soil samples were collected, and actinobacteria were isolated using selective media. All isolates were screened for antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans via the agar well diffusion method. The most potent isolate was subsequently characterized using morphological, biochemical, and 16S rRNA gene sequencing. From 72 distinct isolates, 15 displayed antimicrobial activity. One isolate, designated MGR-17, demonstrated exceptionally potent, broad-spectrum inhibition against all tested pathogens. Based on polyphasic taxonomy, MGR-17 was identified as a potentially novel species of the genus Streptomyces. In conclusion, the rhizosphere of Indonesian mangroves is a fertile source for discovering unique actinobacteria capable of producing novel antibiotics, and the discovery of Streptomyces sp. MGR-17 underscores this potential.
Pengolahan Sampah Organik Sebagai Upaya Peningkatan Ketahanan Iklim Masyarakat Nike Triwahyuningsih; Amrina Yasmine Shafira; Tati Budi Kusmiyarti
ABDIKAN: Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Vol. 4 No. 3 (2025): Agustus 2025
Publisher : Yayasan Literasi Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55123/abdikan.v4i3.6171

Abstract

Community empowerment activities aimed at enhancing the knowledge and skills of the community in managing and processing household organic waste have been conducted in Notoprajan Village, Yogyakarta City. The target of this activity is the PKK members and community groups in the village. The activity was conducted using extension and education methods on waste management, which included waste reduction and handling, followed by practical training on processing organic waste. Through this activity, products have been produced in the form of Ecoenzyme scrub, used cooking oil candles, gkalranulated compost, pasteurized liquid organic fertilizer, tutorial videos, and publications in national journals. It is concluded that this training activity can enhance the knowledge and skills of the residents of Kalurahan Notoprajan in managing and processing organic waste. The impact of the success of this activity is contributing to the direct reduction of organic waste volume, minimizing methane (CH4) production and other greenhouse gas emissions, supporting climate change mitigation efforts at the community level, providing practical skills that can open new economic opportunities for the community, transforming waste into sellable products, and enhancing financial independence, as well as improving climate resilience through increased environmental awareness, independence, and active community participation in maintaining the cleanliness and health of their own environment
Co-Authors Amrina Yasmine Shafira Anna Schneider Chen Mei Tati Budi Kusmiyarti