Soegeng Wahluyo
Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya

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Effect of sucrose and lactose on Streptococcus mutans biofilm adherence Prawati Nuraini; Soegeng Wahluyo; Thania Y. Saroso
Dental Journal (Majalah Kedokteran Gigi) Vol. 59 No. 1 (2026): March
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v59.i1.p74-77

Abstract

Background: In Indonesia, 67.3% of 5-year-old children have dental caries, a prevalent oral illness, with a decayed, missing, and filled teeth index score of 6 or above. Breast milk contains approximately 7% lactose, cow’s milk 4.6%, and first formula milk between 5.4% and 8.2%. Lactose and sugar, however, can aid in the development of Streptococcus mutans, a bacterium linked to dental caries. Purpose: The aim of this study is to examine the differences in S. mutans biofilm adherence after biofilm induction with 5% sucrose and 8% lactose. Methods: Streptococcus mutans biofilms were generated for 24 hours at 37°C using Brain Heart Infusion Broth (BHIB) medium with 5% sucrose and 8% lactose. The optical density value was determined by measuring the amounts of adherent bacteria at 570 nm using a spectrophotometer. The study results were statistically analyzed using variance tests, followed by Tukey’s honest significant difference test (p < 0.05). Results: Streptococcus mutans biofilm induced by sucrose had a higher adherence value than that caused by lactose. There were no significant differences in BHIB with S. mutans and 5% sucrose or 8% lactose (p = 0.100; p < 0.05). Conclusion: Streptococcus mutans induced by 5% sucrose and 8% lactose affected the adherence of bacterial biofilms, but there was no difference in bacterial biofilm adherence between the two compounds. Subsequent research revealed that sucrose and lactose components influenced biofilm adherence.
Management of scurvy in pediatric autism spectrum disorder patient: A case report Alocitta Anindyanari; Diah Ayu Purnama Sari; Delaneira Alvita; Soegeng Wahluyo
Indonesian Journal of Dental Medicine Vol. 9 No. 1 (2026): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v9i1.2026.42-47

Abstract

Background: Scurvy is a disease caused by chronic vitamin C deficiency. It presents with non-specific early signs that can mimic other conditions, such as fatigue, mild fever, and is often followed by dermatologic and musculoskeletal symptoms, anemia, and gingival bleeding. Although rare in the pediatric population, recent studies have reported scurvy can occur in children at risk, including those with neurodevelopmental disorders like autism spectrum disorder (ASD) and even healthy children with restricted or selective feeding. Purpose: This paper aims to report the dental management of scurvy in a pediatric patient with ASD. Case Management: A six-year-old male with ASD was referred to RSGM Universitas Airlangga due to persistent gingival swelling and bleeding for a week, along with pain in the lower limb and difficulty walking. The child’s diet lacked fruits and vegetables. Clinical and laboratory examinations revealed no signs of malignancy or bleeding disorder, though anemia was present. Empirical vitamin C supplementation was given, and oral debridement was performed. Improvements were observed within a week. Conclusion: Identifying scurvy can be challenging due to its nonspecific signs; however thorough history taking, mainly diet history, should be done along with clinical and supporting examinations, hence identification of scurvy can be done as early as possible, and timely treatment can be provided effectively and safely.