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Muhammad Anugerah Alam Waris
Health Polytechnic of Surakarta, Klaten, Central Java, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology

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DETERMINATION OF ANTIOXIDANTS BY DPPH RADICAL SCAVENGING ACTIVITY OF Limonia acidissima L. FRUIT AND EXOCARP EXTRACTS Muhammad Anugerah Alam Waris; Syarifuddin Katjo Arsyad; Suhendriyo Suhendriyo
Jurnal Jamu Kusuma Vol. 5 No. 1 (2025): JURNAL JAMU KUSUMA
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/jurnaljamukusuma.v5i1.46

Abstract

Limonia acidissima is one of the medicinal plants in Indonesia with various bioactive compounds that act as an antioxidant. L. acidissima contains alkaloids, flavonoids, and triterpenoids that function as antioxidants. Antioxidants are compounds that help protect the body from damage to cells by free radicals. To determine the antioxidant activity of a plant, one of the most commonly used measurements is through the capture of free radicals using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. This study has been conducted on the antioxidant activity determination of L. acidissima fruit and exocarp extract with 1,1 Diphenyl-2-picrylhydrazil (DPPH). This study aims to determine the antioxidant activity by observing at the IC50 value contained in the L. acidissima fruit and exocarp extract with DPPH at a wavelength of 478 nm. L. acidissima fruit extract has an IC50 value of 35.190 means antioxidant activity is very strong and L. acidissima exocarp extract has an IC50 value of 35.348 means antioxidant activity is very strong.
THE EFFECT OF GIVING KATE MAS LEAF EXTRACT (Euphorbia heterophylla L.) AS LAXATIVE ON MICE (Mus musculus) Muliana Hafid; Firmansyah Firmansyah; Muhammad Anugerah Alam Waris
Jurnal Jamu Kusuma Vol. 5 No. 1 (2025): JURNAL JAMU KUSUMA
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/jurnaljamukusuma.v5i1.130

Abstract

Kate Mas plant (Euphorbia heterophylla) is a weed plant that grows in humid areas. Traditionally kate mas leaves are used to treat constipation, bronchitis, anti-inflammatory and asthma. The purpose of this study was to determine the effect of giving kate mas leaf extract (Euphorbia heterophylla) as a laxative to mice (Mus musculus). The research design was experimental at the Pharmacology Laboratory, Faculty of Mathematics and Natural Sciences, Pancasakti University. This study used 15 mice which were divided into 5 groups which had been fasted 1 hour before treatment, using loperamide HCl suspension as inducer. The first group was given CMC Na suspension as a negative control, the second group was given dulcolactol syrup as a positive control, groups three, four and five as the treatment group were given katemas leaf ethanol extract with doses of 70 mg/20 g BW, 140 mg/20 g BW and 280 g respectively. mg/20g BW. Data were analyzed using the SPSS application, the Kruskall-Wallis Test followed by testing using the Mann-Whitney Test method to see differences between treatment groups. The results showed that kate mas leaf extract had a laxative effect on mice. The first dose treatment group, namely 70 mg/20 g BW, had the same laxative effect as the positive control group with a significance value (P> 0.05) from these results it could be concluded that kate mas leaf extract had a laxative effect on mice, the most effective treatment group was group three with a dose of 70 mg/20 g BW.
Co-Authors Firmansyah Firmansyah Muliana Hafid Suhendriyo Suhendriyo Syarifuddin Katjo Arsyad