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All Journal Dentino: Jurnal Kedokteran Gigi
Indra Setyawati
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Dentistry

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DESCRIPTION OF ORAL CAVITY MICROBIAL CULTURE IN DENTAL CARIES SUFFERERS Aminah Aminah; Firda Azizah; Indra Setyawati
Dentino: Jurnal Kedokteran Gigi Vol 9, No 2 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i2.20407

Abstract

Background: Dental caries is one of the most common oral health problems worldwide, affecting 573 million children and 2.5 billion adults. Dental caries is a disease that attacks the hard tissues of the tooth, such as cementum, dentin, and enamel. This causes bacterial invasion, pulp death, and spread of infection to periapical tissues, which can cause pain. Purpose: The purpose of this study was to determine the microbial culture picture of the oral cavity of patients with dental caries. Methods: This research is descriptive and the samples in this study amounted to 7 samples of patients with dental caries at Dr. Sitanala Hospital, Tangerang City. The sampling technique in this study used purposive sampling technique. Results: The results of this study found 1 sample (14.3%) of Candida albicans fungus in fungal culture. In addition, 9 samples (64.3%) of Staphylococcus Sp. bacteria, 3 samples (21.4%) of Klebsiella Sp. bacteria, and 2 samples (14.3%) of Bacillus Sp. bacteria were found in the bacteria culture. Conclusion: It has been proven that the oral cavity of dental caries sufferers contains various kinds of microbes, such as the fungus Candida albicans, the bacteria Staphylococcus Sp., the bacteria Klebsiella Sp., and the bacteria Bacillus Sp..
UTILIZATION OF SOYBEAN POWDER AS AN ALTERNATIVE MEDIA FOR THE GROWTH OF ANAEROBIC BACTERIA Aisha Nafa Firdausi; Aminah Aminah; Indra Setyawati
Dentino: Jurnal Kedokteran Gigi Vol 9, No 2 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i2.20408

Abstract

Background:Culture media or microbial growth media is a material consisting of a mixture of nutrients used by microorganisms for growth and reproduction. Anaerobic bacteria can thrive by requiring little or no oxygen for growth and metabolism. Purpose: The purpose to determine the potential of soybean powder as an alternative nitrogen source in Streptococcus mutans bacterial growth media and determine the optimum concentration of soybean powder in alternative media for Streptococcus mutans bacterial growth. Methods: The research method is a laboratory experiment to test soybean powder as an alternative medium for the growth of Streptococcus mutans bacteria using the Candle jar method. The population used is soybean powder with the brand soy flour, while the sample used is Streptococcus mutans bacteria. Results: The results obtained can be known that the average value of colony growth of Streptococcus mutans bacteria in alternative media of soybean powder with a concentration variation of 2 grams was 30 colonies, a concentration variation of 3 grams was 54 colonies, a concentration variation of 4 grams was 40 colonies, and a concentration variation of 5 grams was 72 colonies. From the average value, it can be seen that the growth of Streptococcus mutans on alternative media of soybean powder can grow well, but the most optimum growth is in the concentration variation of 5 grams. Conclusion:Based on the observations obtained, it shows that soybean powder (Glycine max (L.) Merr) can be used as an alternative medium for the growth of Streptococcus mutans bacteria.
Co-Authors Aisha Nafa Firdausi Aminah Aminah Firda Azizah