p-Index From 2021 - 2026
0.408
P-Index
This Author published in this journals
All Journal Jurnal Biogenerasi
Claim Missing Document
Check
Articles

Found 2 Documents
Search

ANALISIS FILOGENETIK GEN atpA PADA GENUS Oryza MENGGUNAKAN PERANGKAT LUNAK MEGA DENGAN PENDEKATAN NEIGHBOR-JOINING Zacki Rafila; Violita; Afifatul Achyar
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/1ptac474

Abstract

This study aims to analyze the phylogenetic relationship of the atpA gene in the genus Oryza using Molecular Evolutionary Genetics Analysis (MEGA) software. The atpA gene, which plays a role in ATP synthesis in chloroplasts, was selected because of its relatively conserved nature and relevance in plant molecular evolution studies. The atpA gene sequences from 13 Oryza species were obtained from the NCBI GenBank database and analyzed using the Neighbor-Joining (NJ) method with the Kimura 2-Parameter model to reconstruct the phylogenetic tree. The reliability of the tree branches was tested through a bootstrap analysis of 10,000 repetitions. The results showed a pattern of kinship consistent with existing taxonomic classifications, in which Oryza sativa tended to form a single clade with Oryza rufipogon as its closest ancestor, while wild species such as Oryza punctata and Oryza officinalis formed a separate and more divergent clade. The relatively high bootstrap values at several branches indicate the reliability of the phylogenetic inferences generated. These findings reinforce the hypothesis that rice domestication involved wild species genetically close to cultivated species and suggest that the atpA gene has the potential to be used as an effective molecular marker in phylogenetic studies of the genus Oryza.
Respon Pertumbuhan Vegetatif Tanaman Padi (Oryza sativa L.) terhadap Aplikasi Kombinasi EM4 dan Trichoderma sp. Anisa tismardi; Filza Yulina Ade; Irma Leilani Eka Putri; Violita
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/hr2s7586

Abstract

Rice (Oryza sativa L.) plays a crucial role as a primary food source in Indonesia, and efforts to improve its growth need to be directed toward sustainable cultivation systems. The use of soil microorganisms, such as Effective Microorganisms-4 (EM4) and Trichoderma sp., is a widely studied alternative due to its ability to improve the condition of other microorganisms obtained from the soil around the roots and support plant growth. This study was conducted to determine the effect of a combination of EM4 and Trichoderma sp. on the vegetative growth of rice plants. The study was conducted from December to February 2026 in Pagambiran Ampalu Village, Lubuk Begalung District, using a completely randomized design (CRD) with six treatments and four replications. Observed parameters included plant height, number of leaves, number of tillers, and plant dry weight. Data were analyzed using analysis of variance (ANOVA) followed by Duncan's test at a 5% significance level. The results showed that the combination of EM4 and Trichoderma sp. improved several