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Violita Violita
State University of Padang

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Analisis Pohon Filogenetik Genus Oryza Berdasarkan Gen matK Yoana Marizky Siregar; Violita Violita; Afifatul Achyar
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/6myncp72

Abstract

The genus Oryza includes both cultivated and wild rice species that play an important role in global food systems and crop improvement. These species also exhibit complex evolutionary patterns, making the study of their phylogenetic relationships essential for understanding domestication and genetic diversity. In this study, phylogenetic relationships within the genus Oryza were analyzed using chloroplast matK gene sequences through a bioinformatics approach. A total of twelve species, representing both cultivated and wild taxa, were selected from the GenBank database. Sequence alignment and tree reconstruction were carried out using MEGA version 11. The phylogenetic tree was generated using the Neighbor-Joining method with the Maximum Composite Likelihood model, and its reliability was assessed through 10,000 bootstrap replications. The results show a clear distinction between cultivated and wild Oryza species. Oryza sativa (Japonica and Indica) is closely associated with O. nivara, supported by high bootstrap values, indicating a strong evolutionary link. Meanwhile, several wild species form separate clades, suggesting earlier divergence within the genus. Overall, the matK gene proves useful for describing phylogenetic relationships at the genus level, although its ability to resolve relationships among closely related species remains limited. These findings contribute to a better understanding of the evolutionary structure of Oryza and highlight the potential importance of wild rice species in future conservation and breeding efforts.
Efek Seed Priming KNO3 terhadap Perkecambahan dan Pertumbuhan Awal Benih Padi Varietas IR 64 pada Beberapa Tingkat Salinitas Puji Ramadhani; Annisa Mardhatillah; Violita Violita
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/hcqyq463

Abstract

Salinity is one of the major limiting factors in rice cultivation, negatively affecting seed germination and early plant growth. One approach that can be taken to improve seed tolerance under saline conditions is seed priming. This study aims to examine the effect of potassium nitrate (KNO3) seed priming on the germination and early growth of IR 64 rice seeds at various salinity levels. This study was an experiment designed using a completely randomized design with a single treatment factor, namely NaCl salinity levels (0, 50, 100, and 150 mM), in which all seeds were treated with 1% KNO3 seed priming prior to stress application. Each treatment was replicated four times. The observed parameters included germination percentage, root length, shoot length, seedling length, and seed vigor index. The data were analyzed using analysis of variance (ANOVA) followed by Duncan’s multiple range test at a 5% significance level. The results showed that KNO3 seed priming was able to maintain the germination percentage of IR 64 seeds at all salinity levels, with values that were not statistically significantly different. However, increasing NaCl concentrations had a significant negative effect on the morphological growth of seedlings, as indicated by a decrease in root length, shoot length, seedling length, and seed vigor index. The treatment without salinity produced the best early growth, while high salinity significantly inhibited seedling growth. Based on these results, it can be concluded that KNO3 seed priming plays an effective role in maintaining the viability of IR 64 rice seeds under saline conditions, but it is not yet fully capable of overcoming the negative effects of salinity on early plant growth.