DEDEN SAPRUDIN
Chemistry Department, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Bogor 16680

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Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16 ANJA MERYANDINI; TRIO HENDARWIN; FAHRRUROZI FAHRRUROZI; ALINA AKHDIYA; DEDEN SAPRUDIN; YULIN LESTARI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 3 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100302

Abstract

Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90oC. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2.5 hours at 90oC. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1.98 mg/mL and 523 μmol/minute/mg, respectivelyKey words: xylanase, xylooligosaccharide, arabinofuranosidase, Streptomyces.