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All Journal Biodiversitas Journal of Biological Diversity
ENDANG ANGGARWULAN
Biology Department, Faculty of Mathematics and Natural Sciences, Sebelas Maret University (UNS), Surakarta 57126
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Potency of Lobak Leaves (Raphanus sativus L. var. hortensis Back) as Anticancer and Antimicrobial Candidates RITA RAKHMAWATI; ENDANG ANGGARWULAN; ESTU RETNANINGTYAS
Biodiversitas Journal of Biological Diversity Vol. 10 No. 3 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100310

Abstract

One of vegetables can preventive cancer and have been used traditionally to cure infection, such as lobak (Raphanus sativus L.). Ineffectiveness antibiotics against microbial infections were still problem until now. Types of antibiotics and anticancer agents from natural resources should be explored and developed. This study was aimed to know toxicity effect and antimicrobial activity of active fractions from lobak leaves. Toxicity study was conducted using Brine Shrimp LethalityTest (BST). Samples were prepared at the concentration of 100, 500, and 1000μg/mL. Antibacterial study against Staphylococcus aureus was conducted using agar-well diffusion method at concentration 30, 40, 50, 60, 70, 80, 100%. Ethyl acetate fraction from methanol extract is the most active that had larger clear zone in S. aureus culture (10,64 mm) and insoluble ethyl acetate fraction from methanol extract is the most active against A. salina (84% death A. salina at 100 μg/mL). Bioactive compounds at active fraction were identified to contain polar compounds.Key words: Raphanus sativus L., BST, Staphylococcus aureus, active fraction
Co-Authors ESTU RETNANINGTYAS RITA RAKHMAWATI