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RUTH MELLIAWATI
Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Cibinong-Bogor 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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The evaluation of carrier material for increasing qualities of gel inoculum for nata de coco RUTH MELLIAWATI
Biodiversitas Journal of Biological Diversity Vol. 9 No. 4 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090403

Abstract

Producion of nata de coco is growing fast, in line with increasing product of biocellulose demand. It is requrire a pure inoculum to reach the best biocellulose product. The aim of this reseach is to evaluate the most appropriate carrier material of gel inoculum for nata de coco. The four carrier material are Carboxy Methyl Cellulose (CMC), Agar, Sagu starch and biocellulose pap and its inoculated by Acetobacter sp. RMG-2 and A. xylinum. After inoculation, then put in the plastic bag (50 g/bag) and stored in 4° C. The texture of gel, population of cell andbiocellulose production were observer in 7 days. The result shown that all matterial were suitable to used as carrier for gel inoculum. Both CMC and biocellulose pap have good texture as standard qualities. Population of A. xylinum was 1.28x109 cfu/mL (in CMC carrier), 1.6x106 cfu/mL (in biocellulose pap carrier) after 15 weeks. The weight of biocellulose production was 500 g/L and 740 g/L medium respectively. While the population of Acetobacter sp. RMG-2 on CMC carrier was 1.79x108 cfu/mL and 7.75x107 cfu/mL on cellulose carrier with the weight of biocellulose production 630 g/L and 775 g/L medium respectively. Thus, the carrier material (CMC and Biocellulose pap) are able to keep bacteria without loss their capability to produce cellulose.Key words: Acetobacter sp. RMG-2, Acetobacter xylinum, carrier material, gel inoculum, nata de coco.
Selection of potential microorganism for sago starch fermentation RUTH MELLIAWATI; ROHMATUSSOLIHAT ROHMATUSSOLIHAT; FERRA OCTAVINA
Biodiversitas Journal of Biological Diversity Vol. 7 No. 2 (2006)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d070201

Abstract

Fermentation process of sago starch for the production of bioproduct requires potential microorganism that have ability to hydrolyze sago starch. The purpose of this research was to get the potential of amylolytic microorganisms for their capability of amyloglucosidase activity and to know the sugar strains of the fermentation result. Eleven amylolytic microorganisms (9 strains of mold and 2 strains of yeast) were obtained from the collection Research Centre for Biotechnology – Indonesian Institute of Sciences (LIPI), Cibinong-Bogor were used. The selection step was carried out based on their capability of starch hydrolysis to reducing sugar. The best result indicates that the production of reducing sugar reached the highest 18.485 ppm and amyloglucosidase activities was 3.583 units by KTU-1 strain. The highest total acid obtained was 5.85 mg/mL by Rhizopus IFO.R5442. The cell biomass was obtained between 0.5 to 1.74 g dry weight/100 mL and pH of the final fermentation (72 h) were 3.57 to 8.38.© 2006 Jurusan Biologi FMIPA UNS SurakartaKey words: sago starch, fermentation, potential microorganism, bioproduct.
Co-Authors FERRA OCTAVINA ROHMATUSSOLIHAT ROHMATUSSOLIHAT