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All Journal Biodiversitas Journal of Biological Diversity
ENNY WIDYATI
Pusat Litbang Hutan dan Konservasi Alam, Bogor 16118
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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The use of sulphate-reducing bacteria in bioremediation of ex-coal mining soil ENNY WIDYATI
Biodiversitas Journal of Biological Diversity Vol. 8 No. 4 (2007)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d080408

Abstract

The most serious impact after exploiting coal by opened peat mining is acid mine drainage phenomenon. This is an oxidation of sulphide minerals by releasing sulphate that generate the environment acidity. This study was aimed to observe the ability of sulphate reducing bacteria (SRB) isolated of sludge paper mills in decreasing the ex-coal mining sulphate content. Before inoculating onto the soil, SRB was incubated in the sterilized organic matter for 4 days. Organic matter inhabited SRB mix with ex-coal mining soil (1:3 v/v). As a control was a series ex-coal mining mixed with non inoculated organic matter (1:3 v/v). The experiment is carried out in randomized complete design in 3 replications, each consist of 5 buckets. All buckets were maintained in saturated water content. Every 5 days for 20 days the sulphate content, pH and Eh were assessed to observe the bioremediation progress. The result shown that SRB was able to reduce 84.25% excoal mining sulphate content in 20 days. In consequence, the soil pH was increased from 4.15 to 6.66 during the process. It is recommended that SRB is prospective to be further developed as a sulphate bioremediation agents on ex-coal mining soil.© 2007 Jurusan Biologi FMIPA UNS SurakartaKey words: bioremediation, ex-coal mining, sulphate reducing bacteria
Formulation of microbes inoculum: AMF, PSB and Rhizobium isolated of ex-coal mining site for Acacia crassicarpa Cunn. Ex-benth seedlings ENNY WIDYATI
Biodiversitas Journal of Biological Diversity Vol. 8 No. 3 (2007)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d080315

Abstract

The shoddier succeed land revegetation particularly caused by least adaptability of the seedlings planted on this site. To encourage their growth and survival rate it can be achieved by means do inoculation with the compatible functional microbes such as rhizobium, Psolubilizing bacteria (PSB) and/or arbuscular-mycorrhiza fungy (AMF). This reserach is aimed to formulate the most compatible inoculant to support the growth of A. crassicarpa seedlings. Compatibility study is carried out in RCB design with 3 replications, each contain 5 seedlings. Height and biomass are accessed to measure the growth responses of the seedlings. The result showed that the best reponse is given by consortia that consist of the three kinds of these microbes. This increase the shoot biomass (137%) compare to the control. The consortia also improved N 164%, P 335% and K 167% in the plant tissues. While pure AMF improved absorption of N plants 80%, P 383% and K 51% compare to the control. It is suggested that to prepare the A. crassicarpa seedlings is better inoculated by consortium ofmicrobes or AMF as a sole inoculant.© 2007 Jurusan Biologi FMIPA UNS SurakartaKey words: AMF, PSB, Rhizobium, revegetation
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