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All Journal Biodiversitas Journal of Biological Diversity
NOVIK NURHIDAYAT
Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Bogor 16002
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Physiological test of Lactobacillus sp. Mar 8 probiotic which encapsulated by using spray dryer to reduce cholesterol TITIN YULINERY; EKO YULIANTO; NOVIK NURHIDAYAT
Biodiversitas Journal of Biological Diversity Vol. 7 No. 2 (2006)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d070205

Abstract

The research on physiology test of Lactobacillus sp. Mar 8 probiotic which encapsulated by using spray dryer to reduce cholesterol has been conducted. The aim of the research was to know the influence of encapsulation process on the Lactobacillus cells to its activities on binding cholesterol, the ability to grow of the bacteria on lower pH 1-6 and the growth on Na-taurocholate at 17 mM concentration. The Emulator used was skimmed milk with 5% and 10% in concentration. The result showed that the bacteria used skimmed milk with 10% concentration was remain stable in growing ability such as growing on GYP medium in lower pH (1-6), growing on sodium taurocholate in 17 mM concentration, and the ability on binding cholesterol. So, encapsulated Lactobacillus can be used as probiotic agent to reduce cholesterol. © 2006 Jurusan Biologi FMIPA UNS SurakartaKey words: probiotik, Lactobacillus sp. Mar 8, encapsulation.
Pigment characterization and lovastatin content of Monascus purpureus isolates ERNAWATI KASIM; SRI ASTUTI; NOVIK NURHIDAYAT
Biodiversitas Journal of Biological Diversity Vol. 6 No. 4 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060406

Abstract

Research on characterization of pigment and lovastatin content of M. purpureus had been done. The aim of the research was to select 19 isolates of M. purpureus which produced the highest lovastatin content, and whether there was any correlation between pigment content and the lovastatin content. The isolates were grown on slant agar of bean sprout extract agar for 14 days. The spores suspension was inoculated to sterile cooked rice IR 42, then incubated for 14 days at 27-32oC. The fermented rice called “angkak”, dried in oven at 40-45oC for two weeks. The dried “angkak” was powdered to measure the content of the pigment and lovastatin. By using spectrophotometer the content of the yellow pigment and the red pigment at 390 nm and 500 nm wavelength respectively, could be detected. The content of lovastatin could be measured by using HPLC. The result showed that all the tested isolates could produce lovastatin compound, the yellow and the red pigment. The highest lovastatin content was 0.92% which produced by JmbA isolate. There was no correlation between the pigment and the lovastatin content. Key words: Monascus purpureus, pigment, lovastatin.
Co-Authors EKO YULIANTO ERNAWATI KASIM SRI ASTUTI TITIN YULINERY