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All Journal Biodiversitas Journal of Biological Diversity
SRI PURWANINGSIH
Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Bogor 16002
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Selection of Rhizobium strain from Wonogiri, Central Java on the growth of soybean (Glycine max L.) on the sand sterile medium in greenhouse SRI PURWANINGSIH
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060305

Abstract

An experiment on the selection of Rhizobium strain from Wonogiri, Central Java on the growth of soybean (Glycine max L.) on the sand sterile medium in green house. The aim of the experiment the selection and potency of the Rhizobium strain to increase the growth of soybean. The experiment was carried out in green house condition in Microbiology Division, Research Center for Biology-LIPI with sterile sand medium. The research design was Completely Randomized Design with three replications for each treatment. The Rhizobium strains used were 1 W (isolated from bean, Vigna radiata), 2 W (isolated from soybean), 3 W (isolated from bean), 4 W (isolated from soybean), 5 W (isolated from soybean), 6 W (isolated from peanut, Arachis hypogaea), 7 W (isolated from peanut), 8 W (isolated from peanut), the controls were uninoculated with Rhizobium strain and without urea fertilizer (K1), uninoculated and with urea fertilizer equal 100 kg/ha (K2). The plants were harvested after 50 days, the variable of investigation were the dry weight of canopy, roots, nodules root, total plants, number of nodules and ‘symbiotic capacity”. The results showed that all of experiment plant which be inoculated with Rhizobium able toform nodule. Strain of 2 W (isolated from soybean) has given the best effects on the growth of soybean.© 2005 Jurusan Biologi FMIPA UNS SurakartaKey words: Rhizobium strain, Glycine max L, sand sterile
The isolation, enumeration, and characterization of Rhizobium bacteria of the soil in Wamena Biological Garden SRI PURWANINGSIH
Biodiversitas Journal of Biological Diversity Vol. 6 No. 2 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060202

Abstract

The eleven soil samples have been isolated and characterized. The aims of the study were to get the pure culture and some data which described about enumeration and especially their characters in relation to the acids and bases reaction in their growth. The isolation of the bacteria use Yeast Extract Mannitol Agar medium (YEMA) while the characterization by using YEMA medium mixed with Brom Thymol Blue and Congo Red indicators respectively. The results showed that eighteen isolates have been isolated which consisted of three low growing and fifteen fast growing bacteria. Two isolates were not indicated Rhizobium and sixteen were Rhizobium. Density of Rhizobiumenumeration was varied which related to soil organic matter content. The enumeration bacteria in YEMA medium were in the range of 0.6 x 105 and 11.6 x 105 CFU /g soil. The highest population was found in soil sample of Wieb vegetation.© 2005 Jurusan Biologi FMIPA UNS SurakartaKeywords: Rhizobium, Wamena Biological Garden, isolation, enumeration, characterization.
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