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SUTARNO SUTARNO
Jurusan Biologi FMIPA Universitas Sebelas Maret Surakarta 57126
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Genetic variation of Anggoli fish (Pristipomoides multidens) based on allozyme patterns ENDANG WIGATI; SUTARNO SUTARNO; HARYANTI HARYANTI
Biodiversitas Journal of Biological Diversity Vol. 4 No. 2 (2003)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d040201

Abstract

The objectives of the research were to study the genetic variation and allozyme band pattern of Pristipomoides multidens from several locations of Indonesian sea based on the pattern of allozyme. Samples of the fish were collected from three geographically different water areas of Bali, Sumbawa and Moluccas. Ten different enzymes, ADH, MDH, LDH, α-GPD,PGM, GPI, IDH, ME, EST and SP were used in this study. Polymorphic loci of genetic variation were in line with the Hardy-Weinberg’s equilibrium. The genetic variation was calculated based on the proportion of polymorphic loci, frequency ofallele, amount of allele per locus, and heterozygosity. The results of this research indicate that from the 10 differentenzymes, 16 loci were detected, and 3 of them were polymorphic (PGM-1, GPI-1 and EST). Population of Moluccas has 2 polymorphic loci (PGM-1 and GPI-1) by proportion of 13%, however, population from Bali and Sumbawa has only 1polymorphic locus (EST-1) with the value of 6%. The allelic number per locus was 1.06 – 1.125, while the observedheterozygosity (D) of the populations was 0.005. The fish population of Moluccas is having better genetic variation than that of population from Bali and Sumbawa. The genetic distance between populations was between 0.002 – 0.005. The closestgenetic distance is between Bali and Sumbawa (D = 0,002), while the longest genetic distance was resulted betweenpopulations of Sumbawa and Moluccas (D = 0.005). Based on the UPGMA cluster analysis for the genetic distances,indicated that there was 2 main geographic groups, (1) Moluccas, and (2) Bali and Sumbawa as single population.© 2003 Jurusan Biologi FMIPA UNS SurakartaKey words: allozyme, genetic variation, Pristipomoides multidens.
Identification of polymorphism on ND-5 mitochondrial DNA fragment of Benggala and Madura cattle with PCR-RFLP technique NEO ENDRA LELANA; SUTARNO SUTARNO; NITA ETIKAWATI
Biodiversitas Journal of Biological Diversity Vol. 4 No. 1 (2003)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d040101

Abstract

The objectives of the research were to detect genetic variations on ND-5 region of mtDNA of Benggala and Madura cattles, and to compare the genetic diversity within or between Madura and Benggala cattle. Genetic variations and its effects on phenotype characters have been studied largely in dairy cattles, but not for beef cattles, especially for Indonesian local cattles. PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) was used to detect polymorphism on ND-5 region of mitochondrial DNA. Polymorphisms were found on ND-5 mitochondrial DNA fragment using HindIII restriction enzyme. This variation were likely due to lost of HindIII restriction site on ND-5 mithocondrial DNA fragment.© 2003 Jurusan Biologi FMIPA UNS SurakartaKey words: ND-5 mitochondrial DNA, genetic variation, PCR-RFLP.
DNA polymorphism at locus-2 of growth hormone gene of Madura cattle AGUS PURWOKO; SUTARNO SUTARNO; NITA ETIKAWATI
Biodiversitas Journal of Biological Diversity Vol. 4 No. 1 (2003)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d040102

Abstract

The objectives of the research were to detect DNA polymorphism at locus 2 of bovine growth hormone gene of Madura cattle and to know its genetic diversity. DNA polymorphisms and their effect on phenotypic traits have been studied widely in dairy cattle but not for beef cattle, especially for Indonesian local cattle. Polymorphism was detected using PCR-RFLP using primer GH-5 and GH-6 for amplifying locus 2 of growth hormone gene. Genetic diversity was analyzed based on the formula of Nei (1973, 1975). DNA polymorphism was found on locus 2 of growth hormone gene using MspI restriction enzyme. This polymorphism may be caused the lost of restriction MspI site. The genetic diversity was 0.4422.© 2003 Jurusan Biologi FMIPA UNS SurakartaKey words: bovine growth hormon gene, PCR-RFLP, polymorphism, Msp I restriction enzyme, Madura Cattle.
Co-Authors AGUS PURWOKO ENDANG WIGATI HARYANTI HARYANTI NEO ENDRA LELANA NITA ETIKAWATI