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All Journal Biodiversitas Journal of Biological Diversity
AFIZUL SAFWAN AZAHARI
Resource Biotechnology Program, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia MICKY VINCENT; HUANG CHAI HUNG; PATRICIA ROWENA MARK BARAN; AFIZUL SAFWAN AZAHARI; DAYANG SALWANI AWANG ADENI
Biodiversitas Journal of Biological Diversity Vol. 19 No. 4 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190412

Abstract

Vincent M, Hung MC, Baran PRM, Azahari AS, Adeni DSA. 2018. Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia. Biodiversitas 19: 1266-1272. The present study was performed to isolate, identify and determine the diversity of oleaginous yeasts from various sources in Kuching, Sarawak (Malaysia). Microscopic observations via light and scanning electron microscope (SEM) indicated that the yeast isolates were in sizes ranging from 2-3 µm in width and 4-8 µm in length, typical of most unicellular ascomycotic fungi. Polymerase Chain Reaction (PCR) and molecular identification performed on the yeast isolates, targeting the D1/D2 region of the 26S rDNA, identified 6 yeast species from the 21 isolates, namely Pichia manshurica (5/21), Candida krusei (8/21), Candida parapsilosis (1/21), Pichia guilliermondii (2/21), Clavispora lusitaniae (1/21) and Kluyveromyces marxianus (4/21). All 21 yeast isolates accumulated intracellular lipids when grown in nitrogen-limited medium, as tested via Sudan IV staining. The present study is the first to document the production of lipids bodies in C. krusei, C. parapsilosis, and C. lusitaniae. Further investigations to assess the growth kinetics, lipid production efficiencies and lipids profiles of these oleaginous yeasts may provide insights into the possible utilization of these isolates for a variety of scientific, technical and industrial applications.
Co-Authors DAYANG SALWANI AWANG ADENI HUANG CHAI HUNG MICKY VINCENT PATRICIA ROWENA MARK BARAN