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All Journal Biodiversitas Journal of Biological Diversity
SYAHRUDDIN SAID
Research Center for Biotechnology, Indonesian Institute of Sciences. Jl. Raya Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Short Communication: Novel Single Nucleotide Polymorphisms (SNPs) in the 5'UTR of Bovine Heat Shock Protein 70 (bHSP70) Gene and its association with Service per Conception (S/C) of Pasundan cattle SYAHRUDDIN SAID; WIDYA PINTAKA BAYU PUTRA
Biodiversitas Journal of Biological Diversity Vol. 19 No. 5 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190504

Abstract

Said S, Putra WPB. 2018. Short Communication: Novel Single Nucleotide Polymorphisms (SNPs) in the 5'UTR of Bovine Heat Shock Protein 70 (bHSP70) Gene and its association with Service per Conception (S/C) of Pasundan cattle. Biodiversitas 19: 1622-1625. Heat stress in the livestock reduces reproductive traits, including of service per conception (S/C). The influence of heat stress in livestock can be handled through molecular selection. One of the candidate genes that is affecting heat stress tolerance is Bovine Heat Shock Protein 70 (bHSP70) gene. This research was carried out to detect the single nucleotide polymorphism (SNPs) in the 5'UTR of bHSP70 gene from 44 heads of Pasundan cows at breeding station (BPPIBT-SP Ciamis, West Java). Research showed that 18 SNPs were detected with sequencing method. The insertion mutation was detected in all animal studied and occurred at position between g.1112 and g.1113. The moderate of polymorphic informative content (PIC) were detected in two SNPs of g.1117G/A and g.1125A/C. Preliminary analysis showed that haplotype of two homozygote genotype combination from both SNPs had better of S/C value of than the others (P<0.01). Moreover, the average of S/C value in heterozygote genotype seems higher than homozygote genotype. However, the further research for clarification of this research results through large number of observation is important. It was concluded that two SNPs of g.1117G/A and g.1125A/C are potential as marker-assisted selection (MAS) for reproductive traits in Pasundan cows because of moderate PIC value.
Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene PASKAH PARTOGI AGUNG; WIDYA PINTAKA BAYU PUTRA; SAIFUL ANWAR; ARI SULISTYO WULANDARI; SYAHRUDDIN SAID; MOCH SYAMSUL ARIFIN ZEIN; ADITYA SUDIRO
Biodiversitas Journal of Biological Diversity Vol. 19 No. 2 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190240

Abstract

Agung PP, Putra WPB, Anwar S, Wulandari AS, Zein MSA, Said S, Sudiro A. 2018. Novel Single Nucleotide Polymorphisms in the Sumba Ongole  (Bos indicus) Growth Hormone Gene. Biodiversitas 19: 676-681. The Sumba Ongole cattle  (Bos indicus) is one of the local Indonesian cattle breeds and has excellent potential to gain higher carcass yield compared to other local cattle breeds in Indonesia. The Growth Hormone  (GH) gene was a potential target for molecular study due to its importance as growth regulating hormone. This study was aimed to determine Single Nucleotide Polymorphism  (SNP) profile of the GH gene in the Sumba Ongole  (SO) cattle using PCR and DNA sequencing methods from 34 individual DNA samples. The results from this study showed that there are 28 SNPs identified within the GH gene; Twenty one SNPs were found to occur in the intron region, 4 SNPs in the exon region, and 3 SNPs in the 3’ end region. There were 17 SNPs in the SO cattle GH gene were caused by novel mutation. The novel SNP g.1395insC was found only in the SO cattle. Moreover, the frequency of the SNP g.1395insC was the highest among other novel SNPs in the SO cattle GH gene. The moderate PIC value  (0.25<PIC<0.50) were found in two novel SNPs i.e. SNP g.1415C>G  (intron 4), and SNP g.1526A>G  (intron 4). The GH gene in the SO cattle was polymorphic and the 17 novel SNPs need to be further validated.
Co-Authors ADITYA SUDIRO ARI SULISTYO WULANDARI MOCH SYAMSUL ARIFIN ZEIN PASKAH PARTOGI AGUNG SAIFUL ANWAR WIDYA PINTAKA BAYU PUTRA