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FARAH FARAHANI
Department of Microbiology, Qom Branch, Islamic Azad University. Qom, Iran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets ZAHRA NOORMOHAMMADI; MARYAM TABAN; FARAH FARAHANI
Biodiversitas Journal of Biological Diversity Vol. 19 No. 5 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190530

Abstract

Noormohammadi Z, Taban M, Farahani F. 2018. Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets. Biodiversitas 19: 1805-1810. Catharanthus roseus L.G. Don, is the essential medicinal plant with considerable attention. This plant is a rich source of terpenoid indole alkaloids (TIAs). The main alkaloids in C. roseus are vinblastine, vincristine, and ajmalicine. The tryptophan decarboxylase (Tdc) and Strictosidine synthase (Str) are key enzymes in TIA biosynthesis. In the present study, Tdc and Str gene expressions, as well as vinblastine production were evaluated in tissue culture regenerated plantlets in 4 groups; control, 60 Gy irradiation, 50 mg/L putrescine and 60 Gy irradiation + 50mg/L putrescine treatments. The results revealed significant increase in Tdc and Str gene expressions in 60 Gy irradiation + 50mg/L putrescine treated plantlets in comparison with control samples by using qPCR methods. HPLC analysis showed a higher amount of vinblastine in 60 Gy + 59 mg/L putrescine treated plantlets. Gamma radiation and putrescine as elicitor and polyamine, respectively, are able to improve vinblastine production in C. roseus.
Co-Authors MARYAM TABAN ZAHRA NOORMOHAMMADI