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AHMAD YUNUS
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36 A, Surakarta 57126, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker MARIA SERVIANA DUE; ARI SUSILOWATI; AHMAD YUNUS
Biodiversitas Journal of Biological Diversity Vol. 20 No. 5 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200534

Abstract

Abstract. Due MS, Susilowati A, Yunus A. 2019. The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker. Biodiversitas 20: 1416-1422. Pisang Ambon (Musa paradisiaca var. sapientum (L.) Kunt.) is one of the horticultural commodities that have high production in Indonesia, making it a superior product. Bananas are propagated vegetatively, which hampers the production of genetic variation and requires a long generation time in the vegetative cycle. This study aimed to determine the diversity of Pisang Ambon induced by gamma radiation of various dosages (10 Gy, 20 Gy and 30 Gy) in vitro based on the ISSR (Inter-Simple Sequence Repeats) marker. The results showed that gamma-ray radiation influenced the morphological properties of plant height, stem diameter, leaf length, leaf width and leaf number based on ANOVA variance analysis. Increased growth occurs in plants with radiation doses of 10 Gy and 20 Gy. The molecular analysis of ISSR uses 6 primers, namely ISSR 835, ISSR 836, ISSR 846, ISSR 847, ISSR 848 and ISSR 855. Of the 6 primers, only 2 primers were able to produce polymorphisms above 50%. A total of 9 bands were produced, in which 8 bands showed polymorphic with a percentage of 90%. The similarity matrix ranged from 0.25-0.93, indicating the level of variation between mutants. The result of dendrogram was divided into two groups with a coefficient distance of 0.47-0.93; plants at a dose of 10 Gy have the highest diversity with the furthest coefficient of diversity distance of 0.47.
Morphological diversity, total phenolic and flavonoid content of Echinacea purpurea cultivated in Karangpandan, Central Java, Indonesia Dwi Fajar Sidhiq; YULI WIDIYASTUTI; DYAH SUBOSITI; BAMBANG PUJIASMANTO; Ahmad Yunus
Biodiversitas Journal of Biological Diversity Vol. 21 No. 3 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210355

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Abstract. Sidhiq DF, Subositi D, Widiyastuti Y, Pujiasmanto B, Yunus A. 2020. Morphological diversity, total phenolic and flavonoid content of Echinacea purpurea cultivated in Karangpandan, Central Java, Indonesia. Biodiversitas 21: 1265-1271. Purple coneflower (Echinacea purpurea (L.) Moench) is a medicinal plant introduced from North America. While it has been introduced to Indonesia long several decades ago. E. purpurea has not been developed as a raw material for herbal medicines in Indonesia as it faces constraints, namely non-uniform production and lack of information on the total phenolic and flavonoid content if cultivated in the country. This study aims to determine the morphological diversity, total phenolic and flavonoid content of three accession E. purpurea cultivated on lowland area (i.e. 493 m asl) in Tegalgede, Karangpandan, Central Java, Indonesia. This study used a Randomized Complete Group Design (RCBD) method with 1 factor, namely 3 accessions of E. Purpurea: A1, A2, A3 each with four replications. Morphological observations, as well as laboratory analysis, were done in this research to collect quantitative and qualitative data. The quantitative data were analyzed using SPSS to test the difference. We found there is significantly different results E. Purpurea accession in term of plant height, wet stover weight, number of leaves, root weight, root length, number of branches. Quantitative observation in the form of extract yield, total phenol content and total flavonoid highest content of extract residue produced by Accession 3 with 7,655%, while the highest phenolic was produced by Accession 1 with 507,619 mg GAE.g -1 extract, and the highest flavonoid was produced by Accession 2 with 313,869 mg QE.g-1 extract. This study concludes that there is morphological diversity of E. purpurea cultivated in Karangpandan with a significant correlation of accession to residue extract, phenolic, and flavonoid content.
Morphological characteristics and flavonoid accumulation of Echinacea purpurea cultivated at various salinity Jessyca Putri Choirunnisa; Yuli Widiyastuti; Amalia Tetrani Sakya; ahmad yunus
Biodiversitas Journal of Biological Diversity Vol. 22 No. 9 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220915

Abstract

Abstract. Choirunnisa JP, Widiyastuti Y, Sakya AT, Yunus A. 2021. Morphological characteristics and flavonoid accumulation of Echinacea purpurea cultivated at various salinity. Biodiversitas 22: 3716-3721. Purple coneflower (Echinacea purpurea (L.) Moench) is an introduced medicinal plant from North America. E. purpurea has high morphological characteristics on stems, leaves and flowers. This plant has not much cultivated as a raw material for traditional medicine in Indonesia due to not much information about flavonoid accumulation of E. purpurea in this country. The purpose of this research was to study morphological characteristics from three accessions of E. purpurea cultivated with various salinity and to select E. purpurea accessions that have high flavonoid accumulation. This study design using a factorial Completely Randomized Design (CRD). The first factor is 3 accessions of E. purpurea (E1; E2; E3). The second factor is 4 levels of CaCl2 (0 ppm; 2500 ppm; 5000 ppm; 10000 ppm). The study was conducted by observing the morphological characteristics of stems, leaves flowers, and herb extract and flavonoid accumulation were analyzed using SPSS. The results demonstrated that morphological characteristics are easy to observed on leaf shape and flower color. The highest herb extract with 10.043% and flavonoid accumulation with 0.510% were in accession 2 with the addition of  CaCl2 concentration of 10000 ppm. This study concludes that there are morphological characteristics of E. purpurea cultivated at various salinity and the highest CaCl2 concentration can increase with significance to herb extract and flavonoid accumulation.
Short Communication: Morphological diversity and the addition of golden snail protein: Its effect on flavonoid content on Echinacea purpurea Wina Chandra Ferdyana; Yuli Widiyastuti; Bambang Pujiasmanto; Amalia Tetrani Sakya; ahmad yunus
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230108

Abstract

Abstract. Ferdyana WC, Widiyastuti Y, Pujiasmanto B, Sakya AT, Yunus A. 2021. Short Communication: Morphological diversity and the addition of golden snail protein: Its effect on flavonoid content on Echinacea purpurea. Biodiversitas 23: 62-66. Echinacea purpurea is a subtropical medicinal plant that contains flavonoids. Flavonoids function as antioxidants and immunomodulators. However, information on the method to increase flavonoid content in E. purpurea is still limited. The golden snail protein increases flavonoid content. The purpose of this study was to determine the best accession and optimal concentration of golden snail protein on biomass production and flavonoid content in E. purpurea. The study used a Factorial Completely Randomized Design (CRD). The first factor was three accessions of E. purpurea (A1, A2, A3); the second factor was 5 levels of the addition of golden snail protein (0 mL/L/week, 10 mL/L/week, 20 mL/L/week, 30 mL/L/week, and 40 mL /L/week). There were differences in morphological characters of three E. purpurea accessions, i.e., stem and the shape of the flower crown. Accession 2 had the highest flavonoid content except for the 10 mL/L/week treatment. The highest increase in flavonoid content in three accessions was obtained in the treatment of 10 mL/L/week gold snail protein by 19% flavonoid content compared to control treatment (0 mL/L/week). The concentration of 0 mL/l/week or control had the lowest flavonoid content.
Co-Authors Amalia Tetrani Sakya Amalia Tetrani Sakya ARI SUSILOWATI BAMBANG PUJIASMANTO Dwi Fajar Sidhiq DYAH SUBOSITI Jessyca Putri Choirunnisa MARIA SERVIANA DUE Wina Chandra Ferdyana Yuli Widiyastuti YULI WIDIYASTUTI