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All Journal Biocaster : Jurnal Kajian Biologi
Syahmi Edi
Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan, Jalan William Iskandar Ps. V, Deli Serdang, Sumatera Utara 20221, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Pengaruh BAP (Benzil Amino Purine) dan Ekstrak Tauge (Phaseolus aureus) terhadap Multiplikasi Tanaman Nilam (Pogostemon cablin Benth.) Secara In Vitro Aprinia Hutagaol; Syahmi Edi
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 1 (2026): January
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i1.1034

Abstract

Patchouli (Pogostemon cablin Benth.) is a promising export commodity, so techniques such as tissue culture are needed to increase its production. This study aims to determine the effect of BAP (Benzyl Amino Purine) and bean sprout extract (Phaseolus aureus) on the multiplication of patchouli (Pogostemon cablin Benth.) in vitro. This study used a factorial Completely Randomized Design (CRD) with 4 levels of BAP concentration (0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm) and 3 levels of bean sprout extract concentration (0%, 12%, 15%), each treatment was repeated 3 times. Data analysis used ANOVA and further testing with 5% DMRT test. The results showed that the administration of BAP significantly affected the increase in the number of shoots, leaves, and roots. BAP concentration of 0.5 ppm gave the best results on the growth of shoots and leaves, but the treatment without BAP actually produced the best growth in the number of roots. The combination of BAP and bean sprout extract significantly affected the number of shoots and leaves. The combination of 0.5 ppm BAP and 15% bean sprout extract produced the highest number of shoots and leaves, producing 2.92 shoots and 4.28 leaves. The interaction between BAP and bean sprout extract creates natural auxin and synthetic cytokinin hormones derived from BAP, which maintain hormonal balance to support patchouli growth.
Analisis Kandungan Fitokimia dan Aktivitas Antioksidan Daun Ciplukan (Physalis angulata L.) Berdasarkan Ketinggian Tempat Tumbuh Rivaldi Ariansah Marpaung; Syahmi Edi
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 1 (2026): January
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i1.1040

Abstract

This study aims to analyze the differences in secondary metabolite content and antioxidant activity in the extract of ground cherry leaves grown in two locations with different altitudes. This study was designed with a descriptive experimental study. The research parameters carried out in the study were secondary metabolite compounds resulting from GC-MS (Gas Chromatography-Mass Spectrometry) analysis and IC50 values ​​were measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The results showed differences in the content of secondary metabolite compounds and IC50 values ​​of antioxidant activity based on the altitude of the growing place. The extract of lowland ground cherry leaves (Kisaran) produced 13 compounds with a tendency towards carbohydrate/sugar derivatives, such as Melezitose. Meanwhile, the extract of highland leaves (Parongil) produced 11 compounds with a tendency towards alkane derivatives, including Phytol. There was no difference in antioxidant activity influenced by the part of the plant and the altitude of the growing place. The Kisaran ground cherry leaf extract with an IC50 value of 54.46 ppm and the Parongil ground cherry leaf extract with an IC50 value of 98.82 ppm are included in the strong antioxidant activity category. Meanwhile, the Kisaran ground cherry fruit extract with an IC50 value of 120.38 ppm and the Parongil ground cherry fruit extract with an IC50 value of 127.65 are included in the moderate antioxidant activity category.
Pengaruh Konsentrasi Ekstrak Bawang Merah (Allium cepa L.) sebagai Zat Pengatur Tumbuh terhadap Pertumbuhan Krisan (Chrysanthemum morifolium) Secara In Vitro Radhika Nabila Ma’rufi Ridwan; Syahmi Edi
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1170

Abstract

Chrysanthemum (Chrysanthemum morifolium) is a major ornamental plant commodity in Indonesia with a production level of up to 459 million stems. The success of in vitro propagation is largely determined by the use of appropriate Plant Growth Regulators (PGRs). This study aimed to evaluate the performance of shallot (Allium cepa L.) extract as a natural PGR, while determining the optimal dose to support chrysanthemum growth in vitro. The study was conducted at the Laboratory of the Horticulture Main Center (BIH) Gedung Johor, Medan, from October 2025 to January 2026, using a Completely Randomized Design (CRD) that included six concentration levels (K0=0; K1=5; K2=10; K3=15; K4=20; and K5=25 mL L⁻¹), with 10 replications. Data analysis was performed using ANOVA-DMRT for parametric data and Kruskal-Wallis–Dunn for non-parametric data. The study results indicated a significant effect (p < 0.01) on all growth parameters. A concentration of 20 mL L⁻¹ was most effective for organogenesis, producing 15.60 leaves, 7.85 roots, and 3.15 shoots; while 5 mL L⁻¹ was superior for elongation, with plantlets reaching 8.99 cm in height. Concentrations above the optimal dose actually inhibited growth due to the accumulation of phytotoxic compounds. These findings provide a more cost-effective and sustainable chrysanthemum propagation protocol as a substitute for synthetic plant growth regulators.
Co-Authors Aprinia Hutagaol Radhika Nabila Ma’rufi Ridwan Rivaldi Ariansah Marpaung