siti samiyarsih
Faculty of Biology, Universitas Jenderal Soedirman. Jl. Dr. Suparno No. 63, Grendeng, Purwokerto, Banyumas 53122, Central Java, Indonesia

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Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents siti samiyarsih; NETTYANI NAIPOSPOS; DIAN PALUPI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 10 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201029

Abstract

Abstract. Samiyarsih S, Naipospos N, Palupi D. 2019. Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents. Biodiversitas 20: 2986-2993. Catharanthus roseus (L.) is an important medicinal plant of the family Apocynaceae used to treat many of the diseases. C. roseus also possess good antioxidant potential. The purpose of this research was 1) to determine the variability of C. roseus based on the morphological, anatomical characters and chlorophyll contents, 2) to know the differences in morphological, anatomical characteristics and chlorophyll contents of C. roseus found in Banyumas Regency. The research used a survey method, with a purposive sampling technique. Preparation of leaf for anatomical analysis was based on paraffin method. The results showed that C. roseus in Banyumas Regency were grouped into eight varieties. The morphological characters observed consisted of 21 characters, 8 quantitative characters, and 13 qualitative characters. Most of the quantitative morphological and anatomical characters were significantly diverse (p <0.05) except for petiole length, corolla number, stomata width and number of trichomata. The eight varieties of C. roseus have different chlorophyll a, chlorophyll b, and total chlorophyll contents but their anthocyanin contents were not significantly different.
Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS siti samiyarsih; NUR FITRIANTO; ELLY PROKLAMASININGSIH; JUWARNO; JUNI SAFITRI MULJOWATI
Biodiversitas Journal of Biological Diversity Vol. 21 No. 4 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210409

Abstract

Abstract. Samiyarsih S, Fitrianto N, Proklamasiningsih E, Juwarno, Muljowati JS. 2020. Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS. Biodiversitas 21: 1332-1344. Catharanthus roseus (L.) G is an important medicinal plant to evaluate the possibility of novel pharmaceuticals since most of the bacterial pathogens are developing resistance against antibiotics. This research aimed to determine the phytochemical diversity of methanol extract of eight cultivars of C. roseus and to evaluate for possible antimicrobial (antifungal and antibacterial) activities. It is the first research to compare phytochemicals and antimicrobial potential among C. roseus cultivars. The compound obtained was screened by Gas Chromatography-Mass Spectrometry (GC-MS) method. While agar-well disc diffusion method was employed to measure antimicrobial activity against Escherichia coli, Staphylococcus aureus, Aspergillus flavus and Aspergillus niger. Based on GC-MS analysis, a total of 18 significantly different metabolite compounds. The abundances of phytochemical compounds (18 classes total) in each cultivar were Dark Pink (66%), Pink (50%), Purple Pink (44%), Pale Pink (27%), White (44%), Milky White (50%), Whitish pink (50%) and Pinkish Red (55%). The leaves extracts showed antimicrobial activity with inhibition zones ranging from 6.40-22.00 mm and 3.35-8.20 mm, respectively. The best antimicrobial activity against E. coli, S. aureus, A. flavus, and A. niger with the zone of inhibition 16.10±1.67 mm, 22.00±0.33 mm, 6.05±0.67 mm and 8.20±0.50 mm respectively by Dark Pink cultivar.