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Lenni Fitri
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala.Jl. Syech Abdurrauf No. 3, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity YEKKI YASMIN; Lenni Fitri; FAUZIAH; FITRI WASLIYAH
Biodiversitas Journal of Biological Diversity Vol. 20 No. 9 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200938

Abstract

Abstract. Yasmin Y, Fitri L, Fauziah, Wasliyah F. 2019. Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity. Biodiversitas 20: 2733-2738. This study aimed to identify bacteria from Apis cerana hive; to determine their antibacterial activity, and cytotoxic effect of hive extract as well as bacterial extract. Identification of bacteria isolated from the hive was carried out based on morphological and biochemical characters. The antibacterial assay of beehive bacteria isolates was done by disk diffusion method against Staphylococcus aureus and Escherichia coli. Toxicity test of beehive extract and beehive bacterial extract were carried out using Brine Shrimp Lethality Test (BSLT) with six final concentrations (32 ppm, 64 ppm, 126 ppm, 250 ppm, 500 ppm, and 1000 ppm). The LC50 value was determined by probit analysis using SPSS. Bacterial isolation showed there were four bacterial isolates from A. cerana hive were the genus Bacillus (BSL1, and BSL3), the genus Micrococcus (BSL2) and the genus Neisseria (BSL4). The antibacterial assay showed that BSL4 isolate has the highest antibacterial activity against S. aureus with the diameter of the inhibitory zone was 32.6 mm. Toxicity test showed that A. cerana hive extract and bacterial ethanol extract from A. cerana hive had LC50 value of 67,744 ppm and 86.985 ppm respectively and categorized as toxic.
Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia Lenni Fitri; KARTINI AMELIA PUTRI; SUHARTONO; YULIA SARI ISMAIL
Biodiversitas Journal of Biological Diversity Vol. 20 No. 10 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201004

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Abstract. Fitri L, Putri KA, Suhartono, Ismail YS. 2019. Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia. Biodiversitas 20: 2802-2808. Proteases are enzymes who catalyze the breakdown of peptide bonds in proteins. This enzyme could be produced from thermophilic bacteria that are able to grow at temperatures of 45-80ºC and are stable to heat. The samples were collected at Ie Seu'um hot spring, Aceh Besar. This study aimed to isolate, to characterize and to determine actinobacteria that were capable of producing protease enzymes. The sampling method in this study was conducted by purposive sampling at temperatures of 50, 60 and 70ºC. Isolation of thermophilic actinobacteria was carried out in Humic Acid Vitamin B (HV) Agar medium and morphological characterization was carried out in Yeast Malt Agar (YMA), Yeast Starch Agar (YSA), Oatmeal Agar (OA) media. Microscopic characterization and measurement of clear zone diameter formed were carried out in skim milk medium. The results showed that one actinobacteria isolate was obtained at a temperature of 50ºC and five isolates at a temperature of 60ºC, meanwhile, no actinobacteria could be obtained at a temperature of 70ºC. A total of 4 isolates obtained were able to produce protease enzymes. The highest Proteolytic Index (IP) value was obtained from IS01 which was 3.8.
Co-Authors FAUZIAH FITRI WASLIYAH KARTINI AMELIA PUTRI SUHARTONO YEKKI YASMIN YULIA SARI ISMAIL