HALIATUR RAHMA
Department of Plant Protection, Faculty of Agriculture, Universitas Andalas. Jl. Lingkar Unand, Limau Manis, Padang 25163, West Sumatra, Indonesia

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Potential of culture filtrate from Trichoderma spp. as biofungicide to Colletotrichum gloeosporioides causing anthracnose disease in chili Nurbailis Nurbailis; AKMAL DJAMAAN; HALIATUR RAHMA; YENNY LISWARNI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 10 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201020

Abstract

Abstract. Nurbailis, Djamaan A, Rahma H, Liswarni Y. 2019. Potential of culture filtrate from Trichoderma spp. as biofungicide to Colletotrichum gloeosporioides causing anthracnose disease in chili. Biodiversitas 20: 2915-2920. Trichoderma spp. have the potential to be used for controlling the airborne pathogenic fungi such as C. gloeosporioides. The purpose of this study was to evaluate the antifungal activity of the culture filtrate of five isolates of Trichoderma spp. (T. harzianum, T. viride, T. koningii, Trichoderma PP2, Trichoderma PP3) against C. gloeosporoides causing anthracnose disease in chili. Culture filtrate of Trichoderma spp. was produced from single culture and dual culture techniques. The design was a Completely Randomized Design with six treatments and four replications. The treatments were culture filtrate from T. harzianum, T. viride, T. koningii, Trichoderma PP2, Trichoderma PP3, and negative control (without culture filtrate from Trichoderma spp.). Parameters observed were: the diameter of the colony, colony coverage, conidial germination, and conidial density. The results of this research showed all the culture filtrate from Trichoderma spp. produced by single and dual culture techniques can inhibit the growth of C. gloeosporioides. The culture filtrate from Trichoderma PP2 and T. koningii were the most potential in inhibiting the growth, conidial density, and conidial germination of C. gloeosporioides.
Diversity of endophytic fungi of rice plants in Padang City, Indonesia, entomopathogenic to brown planthopper (Nilaparvata lugens) TRIZELIA TRIZELIA; HALIATUR RAHMA; MY SYAHRAWATI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 4 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240453

Abstract

Abstract. Trizelia, Rahma H, Syahrawati M. 2023. Diversity of endophytic fungi of rice plants in Padang City, Indonesia, entomopathogenic to brown planthopper (Nilaparvata lugens). Biodiversitas 24: 2384-2392. Various endophytic fungi can live in plant tissues for all or part of their life cycle without causing damage or disease in their host. Several genera of endophytic fungi have been reported to be entomopathogenic and can be used to control insect pests. This study aimed to determine the diversity of endophytic fungi from rice stems that were entomopathogenic against Nilaparvata lugens (Stål, 1854) or Brown Planthopper (BPH). Endophytic fungi were isolated from rice stems of IR42 and Cisokan varieties grown in Padang City, West Sumatra Indonesia. Isolated fungi were first evaluated for their pathogenicity against Tenebrio molitor larvae. Subsequently high pathogenicity showed isolates were tested on nymphs and adults of BPH. The results showed that the entomopathogenic fungi isolated from IR 42 and Cisokan were the members of genus Aspergillus, Trichoderma, and Beauveria. The entomopathogenic fungi from IR 42 were more diverse than Cisokan, and Beauveria sp. was the most common isolate and had a better ability to kill the BPH. The highest mortality of BPH was found in PIR 1.3 isolate, but the mortality was still relatively low (53.33% nymphs and 34.99% adults). Further study is needed to increase the effectiveness of these endophytic fungi against BPH directly or indirectly by inducing resistance.