Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity
Siwi Indarti
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia Siwi Indarti; Alan Soffan; MUHAMMAD MAULANA FARDANI ANDRASMARA
Biodiversitas Journal of Biological Diversity Vol. 21 No. 5 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210533

Abstract

Abstract. Indarti S, Soffan A, Andrasmara MMF. 2020. Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia. Biodiversitas 21: 2068-2073. Hirschmanniella spp. is one of the worldwide plant-parasitic nematodes affecting major losses in rice production and impact up to 25% yield losses especially on irrigated rice. Infection of Hirschmanniella spp. on the root system leading to the typical symptom of red color in the rice rooting system. In order to identify the species variation of Hirschmanniella spp. from the collected samples in Yogyakarta Province of Indonesia, a molecular-based identification using Polymerase Chain Reaction (PCR) method was conducted, complemented by morphological identification technique. PCR based identification was carried out by amplifying the area of ??28S rRNA using universal nematode primer (D2A / D3B) which resulting about 766 bp of amplicon. Blastx analysis from Genbank showed that Cangkringan sample confirmed to be H. mucronata species, while other samples from Banguntapan and Imogiri were H. oryzae species. The Cangkringan samples of H. mucronata have 97.5 % similarities with Belgium sample, forming separate clades with other samples. While both Banguntapan and Imogiri samples have the 99 % similarity with H. oryzae and were located in the same clade, but separated from Cangkringan sample. Morphological identification confirmed both species were distinctly based on the unique characters of the tail tips. H. mucronata therefor is the first report nematode species in Indonesian rice field. Precaution should be designed to prevent the potential distribution of H. mucronata to other areas.
Identification of the root-knot nematode species associated with Carica papaya Stella Nostra Gracia Plena; Siwi Indarti; Ani Widiastuti; Nugroho Susetya Putra
Biodiversitas Journal of Biological Diversity Vol. 23 No. 12 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231216

Abstract

Abstract. Plena SNG, Indarti S, Widiastuti A, Putra NS. 2022. Identification of the root-knot nematode species associated with Carica papaya.Biodiversitas 23: 6212-6217. Root-knot nematodes (Meloidogyne spp.) are important economic pests causing low production and yield losses in horticultural crops. Meloidogyne spp. is widespread and regarded as a major pest since they are found all over the world in a variety of geographic locations, most frequently found in tropical and subtropical climate zones. They are obligate parasites that feed on various plants, including papaya and other horticultural plants. They are root-galling in host plants. Many species of root-knot nematodes may be found in a suitable environment. Therefore, accurate identification of the root-knot nematode species in papaya plants is important for developing and applying control and management measures. Surveys and sampling of nematodes on papaya plants were performed at the Agrotechnology Innovation Center, Universitas Gadjah Mada, the Special Region of Yogyakarta, Indonesia, and its surroundings. Identification was performed both morphologically (based on the perineal pattern of female nematodes) and molecularly (using root-knot nematode species-specific primers). The results revealed that the species of root-knot nematode associated with papaya plants was Meloidogyne javanica based on the perineal pattern of female nematodes, specifically the lateral lines. This finding was also supported by the results of the DNA amplification at 670-bp product using specific primers for M. javanica (FJav/RJav). Consequently, plant protection efforts will need to focus on minimizing M. javanica damage in papaya cultivation.
Abundance and diversity of plant parasitic nematodes associated with vegetable cultivation on various types of organic fertilizers Siwi Indarti; Taryono Taryono; Chandra Wahyu Purnomo; Ayu Suci Wulandari; Rina Maharani
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240241

Abstract

Abstract. Indarti S, Taryono, Purnomo CW, Wulandari AS, Maharani. 2023. Abundance and diversity of plant parasitic nematodes associated with vegetable cultivation on various types of organic fertilizers. Biodiversitas 24: 1010-1016. Planting organic crops is inextricably linked to the presence of Plant-Parasitic Nematodes (PPNs) as a limiting factor. A survey was conducted to determine the abundance and prevalence of PPNs, their correlation with soil chemical properties, and their diversity index in October 2019 at the organic land of the Agrotechnology Innovation Centre, Universitas Gadjah Mada, Kalitirto, Sleman, Special District of Yogyakarta, Indonesia. The organic land was designed as three blocks of different organic fertilizers, including milk sludge fertilizer, cow manure fertilizer, and goat manure fertilizer. The samples were taken by simple randomized sampling method with replications and composited for each treatment in each vegetable plot. Three nematode genera of PPNs were associated with organic vegetable crops in Agrotechnology Innovation Centre, Rotylenchulus sp., Meloidogyne sp., and Helicotylenchus sp. The most common PPNs found was Rotylenchulus sp. with populations of 1388/5 gr roots and 1064/100 mL soil. The other genera, Meloidogynesp. and Helicotylenchus sp. were found with less than 100 nematodes per 5 gr of roots and per 20 gr of soil. C-organic was positively correlated with the Rotylenchulus sp. population. The C/N ratio had a positive effect on the Meloidogyne sp. population, while N-total had a negative effect on the Meloidogyne sp. population. The PPN community diversity index value on the organic land was low (range 0.36-0.37). It indicated that the habitat conditions were relatively homogeneous. It is necessary to carry out integrated management of nematodes to suppress Rotylenchulus sp. population in addition of organic fertilizers on this land.
Co-Authors Alan Soffan Ani Widiastuti Ayu Suci Wulandari Chandra Wahyu Purnomo MUHAMMAD MAULANA FARDANI ANDRASMARA Nugroho Susetya Putra Rina Maharani Stella Nostra Gracia Plena Taryono Taryono