Dermiyati Dermiyati
Department of Soil Science, Faculty of Agriculture, Universitas Lampung. Jl. Prof. Sumantri Brojonegoro, Bandar Lampung 35145, Lampung, Indonesia

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Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome: Dermiyati Dermiyati; Radix Suharjo; Mareli Telaumbanua; Yeyen Ilmiasari; Rully Yosita; RAHMA MEULY ANNISA; ANGGI WINANDA SARI; ANIS PUJI ANDAYANI; DWI MARSENTA YULIANTI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 11 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201126

Abstract

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Ilmiasari Y, Yosita R, Annisa RM, Sari AW, Andayani AP, Yulianti DM. 2019. Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome. Biodiversitas 20: 3315-3321. Palm oil bunches and pineapple rhizomes are abundant in Indonesia, especially in Lampung Province due to widespread agro-industrial company and they are potential to be used as sources for liquid organic fertilizer. This study was aimed to reveal microbial population within liquid organic fertilizer (LF) which was developed from palm oil bunches (PB) and pineapple rhizome (PR) and their role as phosphate solubilizing bacteria (PSB). The capability to solubilize phosphate was recorded from the clear zone area around the bacterial colony which was cultivated on Pikovskaya’s agar medium. The LF was prepared in three kinds of conditions, namely aerobic, anaerobic, and facultative an aerobic. Isolation was performed every 3 days until 24 days after incubations. The results showed that microbial population in the LF from PR was significantly higher than those developed from PB. Totally, 791 bacterial isolates were obtained from the LF; 490 isolates were achieved from PR (153 isolates from aerobic, 188 isolates from facultative anaerobic, 149 from anaerobic conditions) and 301 isolates were collected from PB (96 isolates from aerobic, 112 isolates from facultative anaerobic, 93 isolates from anaerobic conditions). In the case of isolates that were gained from PB, 166 isolates (55.15%) showed capability to degrade phosphate and 135 isolates (44.85%) did not show any capability as PSB. As for isolates obtained from PR, 269 isolates (54.90%) had capability to solubilize phosphate, meanwhile, the other 221 isolates (45.10%) did not show any capability to solubilize phosphate. Among166 isolates of PSB from PB, 60 isolates had very low capability, 55 isolates had low capability, 34 isolates had medium capability, 10 isolates had high capability and only 7 isolates had very high capability. Meanwhile, from 269 isolates of PSB obtained from PR, 135 isolates had very low capability to solubilize phosphate, 84 isolates had low capability to solubilize phosphate, 32 had medium capability to solubilize phosphate, 14 isolates had high capability to solubilize phosphate and 4 isolates produced very high capability as PSB. In total, 11 chosen isolates were selected from LF developed from both PB (7 isolates) and PR (4 isolates). Therefore, palm oil bunches and pineapple rhizomes contained PSB that can be used as LF.
Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions DERMIYATI DERMIYATI; RADIX SUHARJO; MARELI TELAUMBANUA; RULLY YOSITA; ANGGI WINANDA SARI; ANIS PUJI ANDAYANI
Biodiversitas Journal of Biological Diversity Vol. 21 No. 9 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210936

Abstract

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not been carried out optimally. Oil palm empty fruit bunches contain high cellulose content which promotes the development of various types of bacteria with different characteristics. This study was aimed to determine the abundance and the characterization of bacteria that were isolated from extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution under aerobic, anaerobic, and facultative anaerobic conditions. For isolation, the oil palm empty fruit extract suspension was inoculated on media Plate Count Agar Peptone and bacterial abundance was determined. Various bacterial characterization test included bacterial morphology test in the form of color and shape of macroscopic isolates, gram test using KOH 3%, fermentative oxidative test, soft rot test on potato tubers, hypersensitivity test on tobacco plant leaves, and hypo virulent test which was indicated by the presence of symptoms of disease in cucumber sprouts and indicated in the disease severity index (DSI) was carried out. The results of local microorganism solution isolation from oil palm empty fruit bunches obtained as many as 220 bacterial isolates consisting of 84 isolates in aerobic condition, 68 isolates in anaerobic condition, and 68 isolates in facultative anaerobic condition. The highest bacterial abundance in aerobic conditions was equal to 285.60 x 1010 CFU mL-1. Each bacterial isolate had different characteristics. Most of the characteristics of bacteria were turbid white (34.55%), round (69.55%), gram-positive (76.36%), fermentative (89.55%), softrot negative (71.82%), hypersensitive negative (97.27%), and virulent (74.55%).