Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity
SUHARJONO SUHARJONO
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Diversity and potency of indigenous yeast from some palm juices for bioethanol production Trianik Widyaningrum; SUHARJONO SUHARJONO; TRI ARDYATI; AULANNI’AM AULANNI’AM
Biodiversitas Journal of Biological Diversity Vol. 21 No. 1 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210139

Abstract

Abstract. Widyaningrum T, Suharjono S, Ardyati T, Aulanni’am A. 2020. Diversity and potency of indigenous yeast from some palm juices for bioethanol production. Biodiversitas 21: 318-325. Main energy source commonly used by the community comes from fossil energy, especially petroleum. The crisis of energy showed that Indonesia's fossil energy reserves are limited. Based on this fact it is important to develop alternative energy that environmentally friendly and sustain, especially bioethanol. The objective of this research was to diversity and potency of indigenous yeast from palm juice of Arenga pinnata Merr., Cocos nucifera L., Nypa fruticans Wurmb., and Borassus flabellifer L. for bioethanol production. Yeast from juice of the four species of palm was isolated using Yeast Malt Extract Agar (YMEA). Those isolates were screen base on ethanol production in coconut water media. The potential of yeast isolates was identified base on 18S DNA sequence similarity. The four potential isolates of yeast were A3A from A. pinnata, K1A from C. nucifera, N3E from N. fruticans, and S1A from B. flabellifer have highest production of ethanol are 8.6 %, 14.2 %, 9.0 %, and 9.2 % respectively. Four potential isolates of yeast were A3A and N3E, K1A, and S1A have relationship with Saccharomyces cerevisiae NRRL Y-12632T, Pichia manshurica IFO 10726T, and Candida tropicalis ATCC 20615 respectively.
Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia: - Siti Khotimah; Suharjono Suharjono; Tri Ardyati; Yulia Nuraini
Biodiversitas Journal of Biological Diversity Vol. 21 No. 5 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210538

Abstract

Abstract. Khotimah S, Suharjono, Ardyati T, Nurani Y. 2020. Isolation and identification of cellulolytic bacteria at fibric, hemic, and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas 21: 2103-2112. Cellulose degrading bacteria was one of the microbial removers of organic matter contained in the soil into simpler monomers so that it can be utilized by other organisms. The objective of the research was to obtain cellulose-degrading bacteria found on fibric, hemic, and sapric peat in forest and shrubs (oil palm). The bacteria were isolated by pour plate method on 1% CMC media. Selected isolates were assayed quantitatively based on the activity of cellulase enzyme, identified with 16S rDNA. The density of cellulolytic bacteria in the secondary forest peat of fibric, hemic, sapric were 2.1x103 cfu/g, 5.9x104 cfu/g, and 4.9x104 cfu/g whereas, in the area of shrubs/oil palm peat fibric, hemic and sapric 6.9x104 cfu/g, 8.4x104 cfu/g and 3.4x105 cfu/g respectively. There were 19 bacterial isolates that have clear zones around the colony as degradation of cellulose had highest ability to degrade cellulose with clear zones of 5-7 mm. The strain of SB1.1.1 showed highest activity of cellulase enzyme 11.17 U/mL, followed by HH3.1.1 strain and SB2.3 7.83 U/mL. Based on the phylogeny tree, strain SB1.1.1 and HH3.1.1 have the closest kinship relationship with Bacillus cereus with a kinship relationship of 100%, while SB2.3 has the closest kinship relationship with Bacillus stratosphericus with a relationship of 99.85 %.
Exploration of vitamin B12-producing bacteria from Indonesia Eutrophic Lake: A new strategy to improve microalgae biomass production Prabaningtyas Sitoresmi; TRI ARDYATI; SUHARJONO SUHARJONO; CATUR RETNANINGDYAH
Biodiversitas Journal of Biological Diversity Vol. 22 No. 10 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221047

Abstract

Abstract. Prabaningtyas S, Ardyati T, Suharjono, Retnaningdyah C. 2021. Exploration of vitamin B12-producing bacteria from Indonesia Eutrophic Lake: A new strategy to improve microalgae biomass production. Biodiversitas 22: 4538-4544. Biofuel producing autotrophs and heterotrophs organisms are mostly found in freshwaters such as lakes. Preliminary research showed that Ranu Grati has a high diversity of microalgae and bacteria. Microalgae are known as one source of biofuels due to the high content of lipid. The growth of microalgae is strongly influenced by vitamin B12, which is synthesized by bacteria. The role of vitamin B12 in the metabolism of algae is mainly as a co-enzyme for vitamin B12-dependent methionine synthase. The purpose of this research was to observe the bacterial diversity based on NGS analysis, to explore vitamin B12 produced by bacteria from Ranu Grati lake, and also to identify the highest potency of vitamin B12-producing bacteria based on 16S rRNA gene sequencing. The study was carried out by taking a water sample at a depth of 50 cm at five stations. Isolation of total bacterial DNA was carried out using FastDNA Spin Kit for Soil. The metagenomic method of Next Generation Sequencing (NGS) was used as an initial study of bacterial diversity. Vitamin B12-producing bacteria was isolated using PYBG agar (phytone peptone, trypticase peptone, lab-lemco powder, bacto yeast extract, glucose, agar). Isolated bacteria were screened for production of vitamin B12. The potential isolates were identified base on 16S rRNA gene sequence similarity. The results of the metagenomic study showed that the genus of potential bacteria producing vitamin B12 included Bacillus, Pseudomonas, Propionibacterium, Enterobacter, Escherichia, Acinetobacter, and Flavobacterium. The results of screening with PYBG media obtained 30 isolates of vitamin B12 producing bacteria. Ten efficient vitamin B12-producing isolates were identified as Lysinibacillus fusiformis (isolate G2V1), Bacillus cereus group (isolate G2V25, G2V24, G2V13, G2V9, G2V8), Alcaligenes faecalis (isolate G2V22), Delftia acidovorans (isolate G2V14A) and the genus Delftia (isolate G2V19). The isolate G2V1 (Lysinibacillus fusiformis) was the highest producer of vitamin B12 which was able to produce 33,783 ug/mL of vitamin B12 at 4 days incubation time.
Water quality and macroalgal community structure across coastal sites in the Bima Sea, Indonesia NIKMAN AZMIN; CATUR RETNANINGDYAH; SUHARJONO SUHARJONO; WAHYU WIDORETNO
Biodiversitas Journal of Biological Diversity Vol. 27 No. 6 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270606

Abstract

Abstract. Azmin N, Retnaningdyah C, Suharjono, Widoretno W. 2026. Water quality and macroalgal community structure across coastal sites in the Bima Sea, Indonesia. Biodiversitas 27 (6): d270606. https://doi.org/10.13057/biodiv/d270606. Macroalgae are important primary producers in coastal ecosystems and are highly sensitive to changes in environmental conditions. This study aimed to evaluate the physicochemical water quality and macroalgal community structure in aquaculture and non-aquaculture areas at Wane, Rontu, and Tanjung Mas beaches, Bima Regency, West Nusa Tenggara, Indonesia. Community structure was assessed using community indices, the Importance Value Index (IVI), and multivariate analyses. A total of ten macroalgal taxa were identified, with Sargassum sp. exhibiting the highest IVI values across all study sites (59-81), followed by Halimeda micronesica, Ulva lactuca, and Turbinaria sp., which showed intermediate IVI values. Water temperature and pH remained within ranges suitable for supporting marine life, whereas nitrate and orthophosphate concentrations consistently exceeded water quality reference thresholds across all study sites, although no significant differences were detected among locations (p>0.05). The Shannon-Wiener diversity index (H′) ranged from 1.57 to 2.67, with the highest value recorded at the non-aquaculture site in Wane and the lowest at the non-aquaculture site in Tanjung Mas. Multivariate analyses indicated that variation in macroalgal community structure was generally more strongly associated with differences among beaches than with aquaculture and non-aquaculture site categories, although this pattern was most consistently observed at Tanjung Mas Beach. The findings demonstrate that site-specific environmental characteristics play a key role in shaping macroalgal community structure in the Bima Sea. These results highlight the importance of continuous water quality monitoring and location-based coastal management strategies to support macroalgal conservation and the sustainability of tropical coastal ecosystems.
Co-Authors AULANNI’AM AULANNI’AM CATUR RETNANINGDYAH CATUR RETNANINGDYAH NIKMAN AZMIN Prabaningtyas Sitoresmi Siti Khotimah TRI ARDYATI TRI ARDYATI Trianik Widyaningrum WAHYU WIDORETNO Yulia Nuraini