Arsi Arsi
Department of Plant Protection, Faculty of Agriculture, Universitas Sriwijaya. Jl. Raya Palembang-Prabumulih Km 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia

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Characteristics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (Indonesia) and their pathogenicity against oil palm pests Oryctes rhinoceros (Coleoptera: Scarabaeidae) Yulia Pujiastuti; Arsi Arsi; Sofia Sandi
Biodiversitas Journal of Biological Diversity Vol. 21 No. 4 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210403

Abstract

Abstract. Pujiastuti Y, Arsi, Sandi S. 2020. Characteristics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (Indonesia)  and their pathogenicity against oil palm pests Oryctes rhinoceros (Coleoptera: Scarabaeidae). Biodiversitas 21: 1287-1294. Bacillus thuringiensis is a gram-positive, entomopathogenic bacterium that could be isolated from soil and be used to control various plant pests. Oryctes rhinoceros is an important pest in oil palm. Application of B. thuringiensis-based bioinsecticides is an alternative in controlling these pests. The purposes of this study were to isolate and identify B. thuringiensis bacteria from the soil of South Sumatra, production of B. thuringiensis-based bioinsecticides and to test their toxicity to O. rhinoceros larvae. The study was conducted in several cities/districts in the province of South Sumatra. Soil samples were taken from various habitats and B. thuringiensis isolates were grown on NGKG agar media. Among 76 soil samples (6 districts and 2 cities) B. thuringiensis colonies were obtained leading to 24 isolates of B. thuringiensis. Toxicity screening tests for armyworm Spodoptera litura were 55.79% (53 isolates) and their mortality to 25.26% O. rhinoceros larvae (24 isolates). From these isolates whose effectively killed O. rhinoceros larvae, 10 isolates were taken and propagated with Nutrient Broth (NB) and biourine enriched with 5% molasses. Number of spores produced was counted during 24, 48 and 72 hours. Furthermore, a bioassay test was carried out on O. rhinoceros larvae for 7 days. Isolate of KJ3P1 caused the highest mortality of O. rhinoceros larvae after 7 days of observation. SDS Page resulted in KJ3P1 and KJ3R5 isolates showing several bands whose content of various types of protein molecular weight. Isolation of B. thuringiensis in South Sumatra produced 2 isolates potentially to be active ingredients in production of bioinsecticides which were effective in killing O. rhinoceros larvae.
Population of Pentalonia negronervosa Coquerel (Hemiptera: Aphididae) in organs banana cultivars of the Genome AB Arsi Arsi; Titi Tricahayati; Yulia Pujiastuti; Suparman Suparman; Harman Hamidson; Aaliyah Keshya Azriella
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 15 No. 2 (2026): JLSO
Publisher : Research Center for Suboptimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jlso.15.2.2026.810

Abstract

This study aimed to determine the population and morphology of Pentalonia nigronervosa maintained on various AB-genome banana cultivars. The research was conducted using a Factorial Complete Random Design (CRD) with 4 banana cultivars and 3 replications. Indonesia was rich in horticultural crops, including bananas, which were widely cultivated in tropical regions for their high economic value. However, banana cultivation was often constrained by pest infestations, particularly aphids such as P. nigronervosa Coquerel, which can reduce plant productivity and act as vectors of important viral diseases. Therefore, studies on the population dynamics of P. nigronervosa across different organs of banana cultivars in the AB genome group were important for understanding pest distribution and for supporting effective pest management strategies. P. nigronervosa act as pests and vectors that can transmit diseases and result in a decrease in the productivity of banana plants. The population results of P. nigronervosa on plant part factors on days 5, 10, 15, and 20 on the leaf, stalk, and stems. The average population of P. nigronervosa was highest on day 15 at 45.67 in the stem section. The lowest average population on banana plant leaves on days 15 and 20 was 0.00. Population results of P. nigronervosa in the 5th, 10th, 15th, and 20th banana cultivars in the barangan, lilin, susu, and raja bulu cultivars. The average population of P. nigronervosa was highest on the 15th day of the banana susu plant cultivar 36.33. The average population was lowest on 20-day cultivars of bananas barangan and lilin 0.00.